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Vav1 is a guanine nucleotide exchange factor for members of the Rho\/Rac family of GTPases which is activated following TCR stimulation, suggesting that it may transduce TCR signals to the activation of some or all actin\u2010controlled processes. Weshow that Vav1\u2010deficient double\u2010positive thymocytes are less efficient at forming conjugates with APC presenting agonist peptide than wild\u2010type cells are. Furthermore we demonstrate that Vav1 is required for TCR\u2010induced activation of the integrin LFA\u20101, which is likely to explain the defect in conjugate formation. However, once Vav1\u2010deficient cells form a conjugate, the assembly of proteins into an immunological synapse at the conjugate interface is normal. In contrast, thymocyte polarization is defective in the absence of Vav1, as judged by the relocalization of the microtubule\u2010organizing center. These data demonstrate that Vav1 transduces signals to only a subset of cytoskeleton\u2010dependent events at the immunological synapse.<\/jats:p>","DOI":"10.1002\/eji.200323858","type":"journal-article","created":{"date-parts":[[2003,3,18]],"date-time":"2003-03-18T23:11:00Z","timestamp":1048029060000},"page":"790-797","source":"Crossref","is-referenced-by-count":88,"title":["Vav1 transduces TCR signals required for LFA\u20101 function and cell polarization at the immunological synapse"],"prefix":"10.1002","volume":"33","author":[{"given":"Laurence","family":"Ardouin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madelon","family":"Bracke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Mathiot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stamatis N.","family":"Pagakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trisha","family":"Norton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Hogg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor\u2004L.\u2004J.","family":"Tybulewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,2,26]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00120-3"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.8.3909"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/25764"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.170.5.1697"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.5.1461-1477.2000"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80367-2"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.11.2099"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/374467a0"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/374470a0"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)70224-6"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.6.3035"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/374474a0"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)70225-8"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20011663"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.18.10150"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00291-1"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.14.8193"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/351150a0"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199802)28:02<716::AID-IMMU716>3.0.CO;2-E"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.10.1371"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5483.1349"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00299-6"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00326-6"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221458898"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19960616)61:4<554::AID-JCB8>3.0.CO;2-N"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)00074-1"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118651"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.140.3.699"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.8.2.341"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5397.2266"},{"key":"e_1_2_7_31_2","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.4049\/jimmunol.162.3.1367","article-title":"TCR, LFA\u20101, and CD28 play unique and complementary roles in signaling T cell cytoskeletal reorganization.","volume":"162","author":"Sedwick C. 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