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Western blot analysis indicated that PO25 LPS bound to Ra\u2010reactive factor (RaRF), the complex of mannan\u2010binding lectins (MBL) and MBL\u2010associated serine proteases. Binding of RaRF to PO25 LPS led to the activation of C4 component without participation of either C1 or Ig, via the lectin pathway. Relative concentration of RaRF and hemolytic activity in mouse serum decreased rapidly during the process of anaphylactoid reaction. A significant drop of MBL\u2010A, but not MBL\u2010C level was observed. Administrationwith antiserum to RaRF prevented animals from death as a consequence of the inhibition of interaction of RaRF with the carbohydrate target and complement activation. These results indicate that complement\u2010lectin pathway activation is responsible for the anaphylactoid reaction induced with LPS in muramyldipeptide\u2010primed mice. RaRF also activated fibrinogen <jats:italic>in vitro <\/jats:italic>suggesting the involvement of the coagulation system in the process investigated.<\/jats:p>","DOI":"10.1002\/eji.200323949","type":"journal-article","created":{"date-parts":[[2003,9,23]],"date-time":"2003-09-23T22:09:06Z","timestamp":1064354946000},"page":"2842-2852","source":"Crossref","is-referenced-by-count":17,"title":["Role of the complement\u2010lectin pathway in anaphylactoid reaction induced with lipopolysaccharide in mice"],"prefix":"10.1002","volume":"33","author":[{"given":"Anna\u2004St.","family":"\u015awierzko","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maciej","family":"Cedzy\u0144ski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teruo","family":"Kirikae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayasu","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdalena","family":"Klink","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumiko","family":"Kirikae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrzej","family":"Zi\u00f3\u0142kowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evgeny\u2004V.","family":"Vinogradov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaya","family":"Kawakami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,9,16]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.4049\/jimmunol.128.3.1256","article-title":"A new complement\u2010dependent bactericidal factor found in nonimmune mouse sera: specific binding to polysaccharide of Ra chemotype Salmonella.","volume":"128","author":"Ihara I.","year":"1982","journal-title":"J. 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