{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:36:58Z","timestamp":1790192218904,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2003,5,9]],"date-time":"2003-05-09T00:00:00Z","timestamp":1052438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The third complementarity\u2010determining region (CDR) of the TCR \u03b1 and \u03b2 chains forms loops that engage amino acid residues of peptides complexed with MHC. This interaction is central to the specific discrimination of antigenic\u2010peptide\u2013MHC complexes by the TCR. The TCR\u03b2 chain CDR3 loop is encoded by the D\u03b2 gene segment and flanking portions of the V\u03b2 and J\u03b2 gene segments. The joining of these gene segments is imprecise, leading to significant variability in the TCR\u03b2 chain CDR3 loop length and amino acid composition. In marked contrast to other pairing antigen\u2010receptor chains, the TCR \u03b2 and \u03b1 chain CDR3 loop size distributions are relatively narrow and closely matched. Thus, pairing of TCR \u03b1 and \u03b2 chains with relatively similar CDR3 loop sizes may be important for generating a functional repertoire of \u03b1\u2009\u03b2 TCR. Here we show that the TCR\u03b2 chain CDR3 loop size distribution is minimally impacted by TCR\u03b2 chain or \u03b1\u2009\u03b2 TCR selection during thymocyte development. Rather, this distribution is determined primarily at the level of variable\u2010region gene assembly, and is critically dependent on unique features of the V(D)J recombination reaction that ensure D\u03b2 gene segment utilization.<\/jats:p>","DOI":"10.1002\/eji.200323961","type":"journal-article","created":{"date-parts":[[2003,5,27]],"date-time":"2003-05-27T16:03:22Z","timestamp":1054051402000},"page":"1568-1575","source":"Crossref","is-referenced-by-count":41,"title":["T cell receptor CDR3 loop length repertoire is determined primarily by features of the V(D)J recombination reaction"],"prefix":"10.1002","volume":"33","author":[{"given":"Maureen\u2004M.","family":"Hughes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Yassai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John\u2004R.","family":"Sedy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara\u2004D.","family":"Wehrly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching\u2010Yu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osami","family":"Kanagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack","family":"Gorski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry\u2004P.","family":"Sleckman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,5,9]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/302575a0"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.495"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.71.090501.150203"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.10.040192.002043"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00675-X"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.1.323"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.9.4319"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/35014635"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.130190597"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20011803"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.7.3706"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.8.3801"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb03258.x"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.8.12.5459"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90020-4"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(01)00298-9"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00185-4"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.17.1.369"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.15.1.433"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5365.905"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/341742a0"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/46218"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.3109\/08830189609061755"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8356452"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.9.1385"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/6.11.1681"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(02)00326-6"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.20.7856"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1983.tb01097.x"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80053-9"}],"container-title":["European 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