{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T03:22:00Z","timestamp":1775791320812,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,3,23]],"date-time":"2004-03-23T00:00:00Z","timestamp":1080000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Receptors specific for the Fc part of IgG (Fc\u03b3R) are expressed by several cell types and play diverse roles in immune responses. Impaired function of the activating and inhibitory Fc\u03b3Rmay result in autoimmunity. Thus, the modulation of IgG\u2010Fc\u03b3R interaction can be a target for the development of treatments for some autoimmune and inflammatory diseases. This study addresses the localization and functional characterization of linear sequences in human IgG1 which bind to Fc\u03b3RII. Peptides with overlapping sequences derived from the CH2 domain of human IgG1 between P<jats:sup>234<\/jats:sup> and S<jats:sup>298<\/jats:sup> were synthesized and used in binding and functional experiments. Binding of the peptides to Fc\u03b3R was assayed <jats:italic>in vitro<\/jats:italic> and <jats:italic>ex vivo<\/jats:italic>, and peptides foundto interact were functionally tested. The shortest effective peptide was T<jats:sup>256<\/jats:sup>\u2013P<jats:sup>271<\/jats:sup>, which bound to soluble recombinant Fc\u03b3RIIb with <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>=6\u00d710<jats:sup>6<\/jats:sup>\u2004M<jats:sup>\u20131<\/jats:sup>. The biotinylated peptides R<jats:sup>255<\/jats:sup>\u2013P<jats:sup>271<\/jats:sup> and T<jats:sup>256<\/jats:sup>\u2013P<jats:sup>271<\/jats:sup> complexed by avidin exhibited functional activity; they induced Fc\u03b3RIIb\u2010mediated inhibition of the BCR\u2010triggered Ca<jats:sup>2+<\/jats:sup> response of human Burkitt lymphoma cells, and inflammatory cytokine production (TNF\u2010\u03b1 and IL\u20106) by the human monocyte cell line MonoMac. In conclusion, our results suggest that the selected peptides functionally represent the Fc\u03b3RII\u2010binding part of IgG1.<\/jats:p>","DOI":"10.1002\/eji.200324733","type":"journal-article","created":{"date-parts":[[2004,3,23]],"date-time":"2004-03-23T13:41:22Z","timestamp":1080049282000},"page":"1127-1135","source":"Crossref","is-referenced-by-count":13,"title":["Functional mapping of the Fc\u03b3RII binding site on human IgG1 by synthetic peptides"],"prefix":"10.1002","volume":"34","author":[{"given":"D\u00e1vid","family":"Medgyesi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katalin","family":"Uray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krisztina","family":"Sallai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferenc","family":"Hudecz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e1bor","family":"Koncz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jakub","family":"Abramson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Israel","family":"Pecht","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriella","family":"S\u00e1rmay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e1nos","family":"Gergely","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,3,23]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1999.tb01372.x"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.15.1.203"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri856"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3351"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)00038-8"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/35018508"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(84)90088-9"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(86)90059-3"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/332563a0"},{"key":"e_1_2_7_10_2","doi-asserted-by":"crossref","first-page":"2657","DOI":"10.4049\/jimmunol.147.8.2657","article-title":"Human Fc gamma RI and Fc gamma RII interact with distinct but overlapping sites on human IgG.","volume":"147","author":"Lund J.","year":"1991","journal-title":"J. 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