{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T15:00:58Z","timestamp":1773673258154,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2004,8,13]],"date-time":"2004-08-13T00:00:00Z","timestamp":1092355200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The number of T\u2004cells that have undergone proliferation after antigen stimulation <jats:italic>in vivo<\/jats:italic> must be controlled to prevent excessive accumulation of T\u2004cells, autoimmunity, and T\u2004cell neoplasia. We describe here that primary human adenotonsillar memory phenotype CD45R0<jats:sup>+<\/jats:sup> CD4<jats:sup>+<\/jats:sup> T\u2004cells, but not adenotonsillar naive\u2010phenotype CD45RA<jats:sup>+<\/jats:sup> CD4<jats:sup>+<\/jats:sup> T\u2004cells, or peripheral blood naive or memory CD4<jats:sup>+<\/jats:sup> T\u2004cells, express high levels of activation\u2010associated antigens CD38, CD69, CD71, and HLA\u2010DR. These <jats:italic>in vivo<\/jats:italic>\u2010activated CD45R0<jats:sup>+<\/jats:sup> CD4<jats:sup>+<\/jats:sup> T\u2004cells were susceptible to spontaneous and rapid apoptosis <jats:italic>in vitro<\/jats:italic>. Apoptosis could not be inhibited by the disruption of Fas\u2010Fas ligand engagement or by the pan\u2010caspase inhibitor ZVAD. Cross\u2010linking of the T\u2004cell antigen receptor did not rescue cells from apoptosis. Apoptosis could be partially inhibited by the chemokine CXCL12\/SDF\u20101, by IL\u20106, and by the IL\u20102 receptor common \u03b3\u2004chain\u2010signaling cytokines IL\u20102, \u20107, and \u201015. Inhibitors of phosphatidylinositol 3\u2010kinase accelerated apoptosis. We conclude that after <jats:italic>in vivo<\/jats:italic> activation of CD45R0<jats:sup>+<\/jats:sup> CD4<jats:sup>+<\/jats:sup> T\u2004cells, the cells experience a period of intrinsically elevated sensitivity to apoptosis and that multiple external signals control their survival.<\/jats:p>","DOI":"10.1002\/eji.200324761","type":"journal-article","created":{"date-parts":[[2004,8,13]],"date-time":"2004-08-13T18:02:55Z","timestamp":1092420175000},"page":"2771-2780","source":"Crossref","is-referenced-by-count":29,"title":["Human <i>in vivo<\/i>\u2010activated CD45R0<sup>+<\/sup> CD4<sup>+<\/sup> T\u2004cells are susceptible to spontaneous apoptosis that can be inhibited by the chemokine CXCL12 and IL\u20102, \u20106, \u20107, and \u201015"],"prefix":"10.1002","volume":"34","author":[{"given":"Mervi","family":"Pajusto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niina","family":"Ihalainen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jukka","family":"Pelkonen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jussi","family":"Tarkkanen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petri\u2004S.","family":"Mattila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,8,24]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(97)01003-7"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5361.243"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.17.1.221"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/82712"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/373444a0"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/373438a0"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/373441a0"},{"key":"e_1_2_7_8_2","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.4049\/jimmunol.163.3.1315","article-title":"Primed T\u2004cells are more resistant to Fas\u2010mediated activation\u2010induced cell death than naive T\u2004cells.","volume":"163","author":"Inaba M.","year":"1999","journal-title":"J. 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