{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T04:15:25Z","timestamp":1767845725076,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2004,8,18]],"date-time":"2004-08-18T00:00:00Z","timestamp":1092787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Heat shock protein 60 (HSP60) from <jats:italic>Chlamydia pneumoniae<\/jats:italic> was described to trigger <jats:italic>in vitro<\/jats:italic> inflammatory and cytokine responses including TNF and IL\u201012p40. Although it can be found in atherosclerotic plaques of patients, the stimulatory potential of chlamydial and other HSP60 <jats:italic>in vivo<\/jats:italic> is unclear. We now report that chlamydial HSP60 fails to induce TNF expression <jats:italic>in vivo<\/jats:italic>, and significant serum levels of IL\u201012p40 are only found upon intraperitoneal injection of high doses of HSP60 or after intravenous application. Upon purification of chlamydial HSP60 with polymyxin\u2004B\u2010agarose columns, its ability to induce TNF secretion <jats:italic>in vitro<\/jats:italic> is much reduced. However, purified chlamydial HSP60 causes increased serum levels of the CXC chemokines KC and MIP2 <jats:italic>in vivo<\/jats:italic>, as well as a strong accumulation of polymorphonuclear neutrophils (PMN) in the peritoneal cavity upon intraperitoneal challenge. With respect to PMN accumulation, chlamydial HSP60 is more potent than endotoxin or the CpG oligonucleotide\u20041668. The responses observed are completely abolished in Toll\u2010like receptor (TLR)2\/4\u2010double\u2010deficient mice, while single\u2010deficient mice respond almost normally. Furthermore, KC induction and PMN accumulation are largely dependent on MyD88. In conclusion, HSP60 from <jats:italic>C.\u2004pneumoniae<\/jats:italic> triggers inflammatory responses <jats:italic>in vivo<\/jats:italic> that differ from responses induced by endotoxin or CpG oligonucleotides and are dependent on TLR2 and 4.<\/jats:p>","DOI":"10.1002\/eji.200425101","type":"journal-article","created":{"date-parts":[[2004,8,18]],"date-time":"2004-08-18T18:35:58Z","timestamp":1092854158000},"page":"2874-2884","source":"Crossref","is-referenced-by-count":84,"title":["Heat shock protein\u200460 from <i>Chlamydia pneumoniae<\/i> elicits an unusual set of inflammatory responses via Toll\u2010like receptor\u20042 and 4 <i>in vivo<\/i>"],"prefix":"10.1002","volume":"34","author":[{"given":"Clarissa\u2004U.\u2004Prazeres","family":"da Costa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina","family":"Wantia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carsten\u2004J.","family":"Kirschning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk\u2004H.","family":"Busch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuria","family":"Rodriguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hermann","family":"Wagner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Miethke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,8,24]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"crossref","first-page":"3212","DOI":"10.4049\/jimmunol.162.6.3212","article-title":"Human 60\u2010kDa heat\u2010shock protein: a danger signal to the innate immune system.","volume":"162","author":"Chen W.","year":"1999","journal-title":"J. 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