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The aim of this study was to determine if distal regulatory elements contribute to appropriate regulation of the human IL\u20105 (hIL\u20105) gene. Activity of the \u2013507\/+44 hIL\u20105 promoter was compared to expression of the endogenous IL\u20105 gene in PER\u2010117 T cells. The IL\u20105 promoter was not sufficient to reproduce a physiological pattern of IL\u20105 expression. Further, functional analysis of the 5\u2032 and 3\u2032 intergenic regions revealed a number of novel regulatory elements. We have identified a conserved enhancer located approximately 6.2\u2004kb upstream of the hIL\u20105 gene. This region contains two potential GATA\u20103\u2010binding sites and increases expression from the hIL\u20105 promoter by up to ninefold.<\/jats:p>","DOI":"10.1002\/eji.200425279","type":"journal-article","created":{"date-parts":[[2004,11,17]],"date-time":"2004-11-17T16:51:59Z","timestamp":1100710319000},"page":"3633-3643","source":"Crossref","is-referenced-by-count":4,"title":["Distal regulatory elements play an important role in regulation of the human IL\u20105 gene"],"prefix":"10.1002","volume":"34","author":[{"given":"Debra\u2004L.","family":"Urwin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gretchen\u2004T.\u2004F.","family":"Schwenger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David\u2004M.","family":"Groth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin\u2004J.","family":"Sanderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,11,26]]},"reference":[{"key":"e_1_2_7_1_2","first-page":"pp 1","article-title":"The eosinophil and bronchial asthma: Evidence for a critical role of eosinophils in pathophysiology.","author":"Gleich G. 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