{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T04:42:56Z","timestamp":1745815376477},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,3,11]],"date-time":"2005-03-11T00:00:00Z","timestamp":1110499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nearly all hematopoietic receptors are dependent on adaptor proteins for the activation of downstream signaling pathways. The Gads adaptor protein is expressed in many hematopoietic tissues, including bone marrow, lymph node, and spleen. Using intracellular staining, we detected Gads protein in a number cells, including B\u2004cells, T\u2004cells, NK cells, monocytes, and plasmacytoid DC, but not in macrophages, neutrophils, or monocyte\u2010derived DC. In the B\u2004cell compartment, Gads was first expressed after immature B\u2004cells leave the bone marrow and was down\u2010regulated after B\u2004cell antigen receptor (BCR) ligation. Female Gads<jats:sup>\u2013\/\u2013<\/jats:sup> mice had increased numbers of splenic B\u2004cells, as compared to female Gads<jats:sup>+\/+<\/jats:sup> mice, suggesting a role for Gads in B\u2004cell homeostasis. Although B\u2004cell production and turnover of splenic B\u2004cell subsets appeared normal in Gads<jats:sup>\u2013\/\u2013<\/jats:sup> mice, homeostatic proliferation was significantly impaired in Gads<jats:sup>\u2013\/\u2013<\/jats:sup> B\u2004cells. Whereas BCR ligation can induce apoptosis in wild\u2010type transitional stage 1 (T1) B\u2004cells, Gads<jats:sup>\u2013\/\u2013<\/jats:sup> T1 B\u2004cells were resistant to BCR\u2010induced apoptosis. Gads<jats:sup>\u2013\/\u2013<\/jats:sup> B\u2004cells also showed increased BCR\u2010mediated calcium mobilization. We conclude that Gads may have a negative regulatory role in signaling through survival pathways, and is necessary for normal homeostatic proliferation in B\u2004cells.<\/jats:p>","DOI":"10.1002\/eji.200425507","type":"journal-article","created":{"date-parts":[[2005,3,11]],"date-time":"2005-03-11T07:57:35Z","timestamp":1110527855000},"page":"1184-1192","source":"Crossref","is-referenced-by-count":11,"title":["Expression and function of the adaptor protein Gads in murine B\u2004cells"],"prefix":"10.1002","volume":"35","author":[{"given":"Thomas\u2004M.","family":"Yankee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin\u2004E.","family":"Draves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward\u2004A.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,22]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202337"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.24.7273"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.8.1243"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.9.1383"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.11.5805"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni0203-110"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.20.092601.111357"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1057176"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.3.1711"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.1.349"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.8.1151"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.12.6795"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.5.1213"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.595"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2002.18904.x"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.1.75"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.5.2101"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2004.0095.x"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.52.35273"},{"key":"e_1_2_7_20_2","doi-asserted-by":"crossref","first-page":"5827","DOI":"10.4049\/jimmunol.163.11.5827","article-title":"Inhibition of signaling through the B cell antigen receptor by the protooncogene product, c\u2010Cbl, requires Syk tyrosine 317 and the c\u2010Cbl phosphotyrosine\u2010binding domain.","volume":"163","author":"Yankee T. 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