{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:03:28Z","timestamp":1791302608642,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,1,18]],"date-time":"2005-01-18T00:00:00Z","timestamp":1106006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The potent immunomodulator FTY720 elicits immunosuppression via acting on sphingosine 1\u2010phosphate receptors (S1PR), thereby leading to an entrapment of lymphocytes in the secondary lymphoid tissue. To elucidate the potential <jats:italic>in vitro<\/jats:italic> effects of this drug on human monocyte\u2010derived DC, we used low nanomolar therapeutic concentrations of FTY720 and phosphorylated FTY720 (FTY720\u2010P) and investigated their influence on DC surface marker expression, protein levels of S1PR and DC effector functions: antigen uptake, chemotaxis, cytokine production, allostimulatory and Th\u2010priming capacity. We report that both FTY720 and FTY720\u2010P reduce chemotaxis of immature and mature DC. Mature DC generated in the presence of FTY720 or FTY720\u2010P showed an impaired immunostimmulatory capacity and reduced IL\u201012 but increased IL\u201010 production. T\u2004cells cultured in the presence of FTY720\u2010 or FTY720\u2010P\u2010treated DC showed an altered cytokine production profile indicating a shift from Th1 toward Th2 differentiation. In treated immature and mature DC, expression levels for two S1PR proteins, S1P<jats:sub>1<\/jats:sub> and S1P<jats:sub>4,<\/jats:sub> were reduced. We conclude that <jats:italic>in vitro<\/jats:italic> treatment with FTY720 affects DC features that are essential for serving their role as antigen\u2010presenting cells. This might represent a new aspect of the overall immunosuppressive action of FTY720 and makes DC potential targets of further sphingolipid\u2010derived drugs.<\/jats:p>","DOI":"10.1002\/eji.200425556","type":"journal-article","created":{"date-parts":[[2005,1,18]],"date-time":"2005-01-18T17:18:10Z","timestamp":1106068690000},"page":"533-545","source":"Crossref","is-referenced-by-count":107,"title":["The immunomodulator FTY720 interferes with effector functions of human monocyte\u2010derived dendritic cells"],"prefix":"10.1002","volume":"35","author":[{"given":"Hansgeorg","family":"M\u00fcller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Hofer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Kaneider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannes","family":"Neuwirt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Mosheimer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gert","family":"Mayer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcnther","family":"Konwalinka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Heufler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Tiefenthaler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,1,28]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1070238"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(02)00374-6"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C200176200"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200109150-00002"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.00478.x"},{"key":"e_1_2_7_6_2","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1681\/ASN.V1341073","article-title":"First human trial of FTY720, a novel immunomodulator, in stable renal transplant patients.","volume":"13","author":"Budde K.","year":"2002","journal-title":"J. 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