{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T01:23:32Z","timestamp":1792718612585,"version":"4.5.0"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,4,7]],"date-time":"2005-04-07T00:00:00Z","timestamp":1112832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Immunization of mice with live or heat\u2010killed<jats:italic> Listeria monocytogenes<\/jats:italic> (HKLM) efficiently primes pathogen\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2004cells. T lymphocytes primed by HKLM, however, undergo attenuated proliferation and do not fully differentiate. Thus, only infection with live bacteria induces long\u2010term, CD8<jats:sup>+<\/jats:sup> T\u2004cell\u2010mediated protective immunity. In this study we demonstrate that live and heat\u2010killed bacteria, while both associating with Mac\u20103<jats:sup>+<\/jats:sup>CD11b<jats:sup>hi<\/jats:sup> cells, localize to distinct splenic areas following intravenous inoculation. While HKLM localize to the marginal zone and the splenic red pulp, live <jats:italic>L. monocytogenes<\/jats:italic> are carried to the T\u2004cell zone of splenic white pulp. Despite these differences, <jats:italic>in vivo<\/jats:italic> depletion of CD11c\u2010expressing cells prevents priming of naive T\u2004cells by either HKLM or live <jats:italic>L. monocytogenes<\/jats:italic>. Analysis of CD11c<jats:sup>hi<\/jats:sup> dendritic cells (DC) reveals that infection with live <jats:italic>L. monocytogenes<\/jats:italic> induces higher levels of CD40, CD80 and CD86 expression than immunization with HKLM. Our results suggest that CD8<jats:sup>+<\/jats:sup> T\u2004cell priming following HKLM immunization or live infection is mediated by DC and that the disparate outcomes of priming can be attributed to suboptimal conditioning of DC in the absence of live, cytosol\u2010invasive bacteria.<\/jats:p>","DOI":"10.1002\/eji.200526024","type":"journal-article","created":{"date-parts":[[2005,4,7]],"date-time":"2005-04-07T14:47:12Z","timestamp":1112885232000},"page":"1463-1471","source":"Crossref","is-referenced-by-count":78,"title":["Distinct <i>in vivo<\/i> dendritic cell activation by live versus killed <i>Listeria monocytogenes<\/i>"],"prefix":"10.1002","volume":"35","author":[{"given":"Eric","family":"Muraille","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rielle","family":"Giannino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Guirnalda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Leiner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric\u2004G.","family":"Pamer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregoire","family":"Lauvau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,4,22]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.3949\/ccjm.66.6.375"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.116.3.381"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2478(98)00130-8"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.1.259"},{"key":"e_1_2_7_5_2","doi-asserted-by":"crossref","first-page":"679","DOI":"10.4049\/jimmunol.156.2.679","article-title":"Listeria monocytogenes induces apoptosis of infected hepatocytes.","volume":"156","author":"Rogers H. 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