{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T19:55:18Z","timestamp":1772308518101,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,3,21]],"date-time":"2006-03-21T00:00:00Z","timestamp":1142899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chronic beryllium disease (CBD) is characterized pathologically by granulomatous inflammation in the lung, composed of a large core of epithelioid cells surrounded by a dense shell of CD4<jats:sup>+<\/jats:sup> T\u2004cells. Using beryllium\u2010specific CD4<jats:sup>+<\/jats:sup> T\u2004cell lines derived from the bronchoalveolar lavage (BAL) fluid of CBD patients, we show that purified CD4<jats:sup>+<\/jats:sup> T\u2004cells produced significant amounts of IFN\u2010\u03b3 and TNF\u2010\u03b1 upon exposure to beryllium in the absence of antigen\u2010presenting cells (APC). However, unlike BAL T\u2004cells stimulated by beryllium in the presence of APC, self\u2010presentation by BAL T\u2004cells did not induce detectable IL\u20102 production, and in its absence these activated T\u2004cells die from programmed cell death. Resting BAL CD4<jats:sup>+<\/jats:sup> T\u2004cells constitutively express high levels of HLA\u2010DP, lymphocyte function\u2010associated antigen\u20041 (LFA\u20101) and ICAM\u20103. When stimulated with beryllium\/APC, the adhesion molecule ICAM\u20101 was up\u2010regulated, as well as several costimulation molecules including CD28, OX\u201040 (CD134), 4\u20101\u2010BB (CD137) and B7\u20101 (CD80). Notably, CD28 was not up\u2010regulated during self\u2010presentation by BAL T\u2004cells, and these cells do not express OX\u201040L, suggesting that lack of appropriate costimulation was responsible for programmed cell death observed upon beryllium self\u2010presentation. Restricting anti\u2010MHC class\u2004II mAb completely eliminated beryllium\u2010induced T\u2004cell proliferation during self\u2010presentation and significantly reduced IFN\u2010\u03b3 and TNF\u2010\u03b1 production. Our data demonstrate for the first time that self\u2010presentation by BAL T\u2004cells in response to beryllium can occur <jats:italic>ex vivo<\/jats:italic>, in the absence of professional APC, with a specific dependence on T\u2004cell\u2010expressed MHC class\u2004II molecules and exogenous IL\u20102 for survival.<\/jats:p>","DOI":"10.1002\/eji.200526075","type":"journal-article","created":{"date-parts":[[2006,3,21]],"date-time":"2006-03-21T14:48:31Z","timestamp":1142952511000},"page":"930-939","source":"Crossref","is-referenced-by-count":10,"title":["Self\u2010presentation of beryllium by BAL CD4<sup>+<\/sup> T\u2004cells: T\u2004cell\u2013T\u2004cell interactions and their potential role in chronic beryllium disease"],"prefix":"10.1002","volume":"36","author":[{"given":"Andrew\u2004P.","family":"Fontenot","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David\u2004M.","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan\u2004K.","family":"Chou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas\u2004G.","family":"Mack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorian","family":"LaTocha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur\u2004A.","family":"Vandenbark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory\u2004G.","family":"Burrows","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,3,29]]},"reference":[{"key":"e_1_2_7_1_2","first-page":"pp 435","article-title":"Beryllium disease. In Schwarz, M. I. and King, T. E., Jr (Eds.)","author":"Newman L. S.","year":"2003","journal-title":"Interstitial lung disease. 4th Edn. B. C. Decker Inc., Hamilton"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.150.1.8025739"},{"key":"e_1_2_7_3_2","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.4049\/jimmunol.163.2.1019","article-title":"Identification of pathogenic T\u2004cells in patients with beryllium\u2010induced lung disease.","volume":"163","author":"Fontenot A. P.","year":"1999","journal-title":"J. Immunol."},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8105536"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0274(199710)32:4<337::AID-AJIM3>3.0.CO;2-R"},{"key":"e_1_2_7_6_2","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.4049\/jimmunol.163.3.1647","article-title":"Differential susceptibilities to chronic beryllium disease contributed by different Glu69 HLA\u2010DPB1 and \u2010DPA1 alleles.","volume":"163","author":"Wang Z.","year":"1999","journal-title":"J. Immunol."},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.12.6910"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2005.08.004"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.165.6.2104002"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-6-94"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.10.7029"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-108-5-687"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.220430797"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.18.4.2981"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215846"},{"key":"e_1_2_7_16_2","first-page":"267","article-title":"Beryllium disease screening in the ceramics industry: Blood test performance and exposure\u2010disease relations.","volume":"35","author":"Kreiss K.","year":"1993","journal-title":"J. Occup. Med."},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/148.4_Pt_1.985"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.5.3549"},{"key":"e_1_2_7_19_2","doi-asserted-by":"crossref","first-page":"518","DOI":"10.4049\/jimmunol.158.1.518","article-title":"Beryllium induces IL\u20102 and IFN\u2010\u03b3 in berylliosis.","volume":"158","author":"Tinkle S. S.","year":"1997","journal-title":"J. Immunol."},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/00043764-198907000-00011"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(91)90300-D"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1097\/00043764-198303000-00012"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/139.6.1479"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200318317"},{"key":"e_1_2_7_25_2","doi-asserted-by":"crossref","first-page":"2675","DOI":"10.4049\/jimmunol.152.6.2675","article-title":"Naive versus memory CD4 T\u2004cell response to antigen. Memory cells are less dependent on accessory cell costimulation and can respond to many antigen\u2010presenting cell types including resting B\u2004cells.","volume":"152","author":"Croft M.","year":"1994","journal-title":"J. Immunol."},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0952-7915(94)90123-6"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1999.0320"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.1.265"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.02.02232001"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.7.4316"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1385\/IR:23:1:01"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830220812"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830260411"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830201015"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.101130498"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.19.10782"},{"key":"e_1_2_7_37_2","first-page":"990","article-title":"Clinical application of beryllium lymphocyte proliferation testing.","volume":"10","author":"Barna B. P.","year":"2003","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.2000.00933.x"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0198-8859(01)00261-0"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.200526075","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.200526075","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T22:50:11Z","timestamp":1697151011000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.200526075"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,3,29]]},"references-count":39,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,4]]}},"alternative-id":["10.1002\/eji.200526075"],"URL":"https:\/\/doi.org\/10.1002\/eji.200526075","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,3,29]]}}}