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In this study, ZAP\u201070 was detected in normal B cells from human tonsils, but not from peripheral blood. The cDNA sequence of B cell ZAP\u201070 was the same as that in T cells. Germinal center B cells and plasma cells had a substantial proportion of ZAP\u201070<jats:sup>+<\/jats:sup> cells, while memory and follicular mantle B cells, which contain low numbers of activated B cells, expressed relatively little ZAP\u201070. A cell fraction of IgD<jats:sup>+<\/jats:sup>, CD38<jats:sup>+<\/jats:sup> B cells, which are comprised of many <jats:italic>in vivo<\/jats:italic> activated B cells, exhibited the highest levels of ZAP\u201070. Density gradient fractionation of tonsil B cells confirmed that ZAP\u201070 was not expressed by resting B cells, but was expressed by buoyant, <jats:italic>in vivo<\/jats:italic> activated B cells. In these B cells, the expression of ZAP\u201070 correlated with that of CD38 and not with that of CD5, a hallmark of B\u2010CLL cells. B\u2010CLL cells are activated cells and their ZAP\u201070 expression reflects a normal property of activated B cells populations rather than a neoplastic aberration.<\/jats:p>","DOI":"10.1002\/eji.200526355","type":"journal-article","created":{"date-parts":[[2006,2,15]],"date-time":"2006-02-15T16:20:39Z","timestamp":1140020439000},"page":"558-569","source":"Crossref","is-referenced-by-count":58,"title":["B lymphocytes in humans express ZAP\u201070 when activated <i>in vivo<\/i>"],"prefix":"10.1002","volume":"36","author":[{"given":"Giovanna","family":"Cutrona","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Colombo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"Matis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Reverberi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariella","family":"Dono","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Tarantino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Chiorazzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manlio","family":"Ferrarini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,2,28]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90598-7"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00082-7"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324309"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.11.1639"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-06-1683"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(94)90009-4"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI3009"},{"key":"e_1_2_7_8_2","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1182\/blood.V94.6.1840","article-title":"Ig V gene mutation status and CD38 expression as novel prognostic indicators in chronic lymphocytic leukemia.","volume":"94","author":"Damle R. 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