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Although reduced activation is due to decreased hexose monophosphate shunt activity, the mechanism enhancing basal oxidant levels is unknown. We hypothesize that myeloperoxidase (MPO) trafficking affects the basal oxidant release by maternal neutrophils. Immunofluorescence microscopy has demonstrated MPO at the surface of pregnancy neutrophils, whereas non\u2010pregnancy cells do not exhibit surface MPO. Adherent pregnancy neutrophils were characterized by high\u2010amplitude metabolic oscillations, which were blocked by MPO inactivation. Conversely, metabolic oscillatory amplitudes of control neutrophils were heightened by incubation with PMA or exogenous MPO. Importantly, MPO decoration of cell surfaces and high\u2010amplitude metabolic oscillations were observed for neutrophils from pregnant but not from non\u2010pregnant mice. However, cells from pregnant MPO knockout mice did not exhibit MPO expression or high\u2010amplitude metabolic oscillations. Unstimulated neutrophils from pregnant women were found to release reactive oxygen metabolites (ROM) and reactive nitrogen intermediates (RNI), but cells from non\u2010pregnant women did not. MPO inhibition returned ROM and RNI formation to non\u2010pregnant levels. Hence, MPO trafficking influences metabolic activity and oxidant production in pregnancy.<\/jats:p>","DOI":"10.1002\/eji.200535391","type":"journal-article","created":{"date-parts":[[2006,5,10]],"date-time":"2006-05-10T13:24:14Z","timestamp":1147267454000},"page":"1619-1628","source":"Crossref","is-referenced-by-count":27,"title":["Myeloperoxidase accumulates at the neutrophil surface and enhances cell metabolism and oxidant release during pregnancy"],"prefix":"10.1002","volume":"36","author":[{"given":"Andrei\u2004L.","family":"Kindzelskii","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea\u2004J.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimmy","family":"Espinoza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuyo","family":"Maeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuaki","family":"Aratani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Romero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard\u2004R.","family":"Petty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,5,24]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"crossref","first-page":"5436","DOI":"10.4049\/jimmunol.155.11.5436","article-title":"The effect of pregnancy on polymorphonuclear leukocyte function.","volume":"155","author":"Crouch S. 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