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After administration of STxB chemically coupled to OVA (STxB\u2010OVA) in mice, we showed that the immunodominant OVA<jats:sub>257\u2013264<\/jats:sub> peptide restricted by K<jats:sup>b<\/jats:sup> molecules is specifically presented by CD11c<jats:sup>+<\/jats:sup>CD8\u03b1<jats:sup>\u2013<\/jats:sup> DC, some of them displaying a mature phenotype. Using mice carrying a transgene encoding a diphtheria toxin receptor (DTR) under the control of the murine CD11c promoter, which allows inducible ablation of DC, we showed that DC are required for efficient priming of CTL after STxB\u2010OVA vaccination. Immunization of mice with STxB\u2010OVA induced OVA\u2010specific CD8<jats:sup>+<\/jats:sup> T cells detected <jats:italic>ex vivo<\/jats:italic>; these cells were long lasting, since they could be detected even 91\u2004days after the last immunization and were composed of both central and memory T cells. Vaccination of mice with STxB\u2010OVA and STxB coupled to E7, a protein derived from HPV16, inhibited tumor growth in prophylactic and therapeutic experiments. This effect was mainly mediated by CD8<jats:sup>+<\/jats:sup> T cells. STxB therefore appears to be a powerful carrier directly targeting DC <jats:italic>in vivo<\/jats:italic>, resulting in a strong and durable CTL response associated with tumor protection.<\/jats:p>","DOI":"10.1002\/eji.200535443","type":"journal-article","created":{"date-parts":[[2006,3,28]],"date-time":"2006-03-28T10:46:23Z","timestamp":1143542783000},"page":"1124-1135","source":"Crossref","is-referenced-by-count":87,"title":["The Shiga toxin B\u2010subunit targets antigen <i>in vivo<\/i> to dendritic cells and elicits anti\u2010tumor immunity"],"prefix":"10.1002","volume":"36","author":[{"given":"Benoit","family":"Vingert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Adotevi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Patin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Protul","family":"Shrikant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludovic","family":"Freyburger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl","family":"Eppolito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Sapoznikov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Amessou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Quintin\u2010Colonna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolf\u2004Herman","family":"Fridman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludger","family":"Johannes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Tartour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,4,25]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri746"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.231606698"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00352-2"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-01-0380"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00448-2"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.1503"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.5.1517"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.11.1669"},{"key":"e_1_2_7_9_2","first-page":"6451","article-title":"Immune and clinical responses in patients with metastatic melanoma to CD34(+) progenitor\u2010derived dendritic cell vaccine.","volume":"61","author":"Banchereau J.","year":"2001","journal-title":"Cancer Res."},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1039"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030448"},{"key":"e_1_2_7_12_2","first-page":"12","article-title":"Effective migration of antigen\u2010pulsed dendritic cells to lymph nodes in melanoma patients is determined by their maturation state.","volume":"63","author":"De Vries I. 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