{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T16:19:00Z","timestamp":1743265140472},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T00:00:00Z","timestamp":1161129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Epicutaneous sensitization with a protein antigen was demonstrated to induce a predominant type 2 CD4 T cell response with high IgE production in mice. On the other hand, its CD8 T cell responses have not been addressed probably partly because of the generally accepted concept that cross\u2010priming of soluble protein is an inefficient process. Here, we used an established patch\u2010applied murine model to demonstrate that cross\u2010priming with an epicutaneously introduced soluble protein antigen, though inefficient, generated mainly Tc1 cells, but not Tc2 cells. In the presence of an irritant or hapten, the efficiency of this cross\u2010priming process could be enhanced and more Tc1 cells were generated. CpG oligonucleotides also promote the generation of Tc1 cells. In contrast, lipopolysaccharide and poly (inosinic\u2010cytidylic) acid [poly (I:C)] have no effect. Together, these results provide supportive evidence of the epicutaneous sensitization of human cutaneous lymphocyte\u2010associated antigen\u2010positive CD8 T cells found in the peripheral blood or tissues of patients. The surprising observation of the type 1 character of the generated CD8 T cells will also help us to better understand the complicated pathogenesis of atopic and cutaneous inflammatory diseases.<\/jats:p>","DOI":"10.1002\/eji.200535770","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T12:10:25Z","timestamp":1161173425000},"page":"2904-2911","source":"Crossref","is-referenced-by-count":10,"title":["Cross\u2010priming with an epicutaneously introduced soluble protein antigen generates Tc1 cells"],"prefix":"10.1002","volume":"36","author":[{"given":"Li\u2010Fang","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chih\u2010Jung","family":"Hsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shi\u2010Chuen","family":"Miaw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsien\u2010Ching","family":"Chiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching\u2010Yi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsin\u2010Su","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,30]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(98)01301-2"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.47"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/32183"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00388-6"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020295"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.9.5327"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.10.6099"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1147"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-003-0145-z"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00454.x"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215753"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2230.1998.00340.x"},{"key":"e_1_2_7_13_2","doi-asserted-by":"crossref","first-page":"580","DOI":"10.4049\/jimmunol.160.2.580","article-title":"Ovalbumin\u2010specific, MHC class I\u2010restricted, \u03b1\u03b2\u2010positive, Tc1 and Tc0 CD8+ T cell clones mediate the in vivo inhibition of rat IgE.","volume":"160","author":"MacAry P. A.","year":"1998","journal-title":"J. Immunol."},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2002.123233"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.4.1719"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.4.2549"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425018"},{"key":"e_1_2_7_18_2","first-page":"13","article-title":"CLA+ T cells in cutaneous diseases.","volume":"14","author":"Santamaria\u2010Babi L. F.","year":"2004","journal-title":"Eur. J. Dermatol."},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.5.1935"},{"key":"e_1_2_7_20_2","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.4049\/jimmunol.150.3.1122","article-title":"Control of lymphocyte recirculation in man II. Differential regulation of the cutaneous lymphocyte\u2010associated antigen, a tissue\u2010selective homing receptor for skin\u2010homing T cells.","volume":"150","author":"Picker L. J.","year":"1993","journal-title":"J. Immunol."},{"key":"e_1_2_7_21_2","doi-asserted-by":"crossref","first-page":"4079","DOI":"10.4049\/jimmunol.156.11.4079","article-title":"Epicutaneous exposure of protein antigen induces a predominant Th2\u2010like response with high IgE production in mice.","volume":"156","author":"Wang L.\u2010F.","year":"1996","journal-title":"J. Immunol."},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1647"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-004-0174-2"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1098"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00141-5"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.22411.x"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.8.4102"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0818-9641.2004.01285.x"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-11-4240"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/82161"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.3.889"},{"key":"e_1_2_7_32_2","doi-asserted-by":"crossref","first-page":"351","DOI":"10.4049\/jimmunol.152.1.351","article-title":"Inhibition of IgE production and normalization of airways responsiveness by sensitized CD8 T cells in a mouse model of allergen\u2010induced sensitization.","volume":"152","author":"Renz H.","year":"1994","journal-title":"J. Immunol."},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030266"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI21060"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.03.015"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI9199"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.200535770","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.200535770","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T19:27:34Z","timestamp":1697138854000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.200535770"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10,30]]},"references-count":36,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1002\/eji.200535770"],"URL":"https:\/\/doi.org\/10.1002\/eji.200535770","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10,30]]}}}