{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:32:51Z","timestamp":1791304371481,"version":"4.3.1"},"reference-count":63,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,4,11]],"date-time":"2007-04-11T00:00:00Z","timestamp":1176249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Limited studies have addressed the ability of \u03b3\u03b4 T cells to become memory populations. We previously demonstrated that WC1.1<jats:sup>+<\/jats:sup> \u03b3\u03b4 T cells from ruminants vaccinated with killed <jats:italic>Leptospira borgpetersenii<\/jats:italic> proliferate and produce IFN\u2010\u03b3 in recall responses. Here we show that this response is dependent upon antigen\u2010responsive CD4 T cells, at least across transwell membranes; this requirement cannot be replaced by IL\u20102. The response was also dependent upon <jats:italic>in vivo<\/jats:italic> priming, since \u03b3\u03b4 T cells from leptospira vaccine\u2010naive animals did not respond to antigen even when co\u2010cultured across membranes from antigen\u2010responsive PBMC. \u03b3\u03b4 T cells were the major antigen\u2010responding T cell population for the first 4\u2004wks following vaccination and replicated more rapidly than CD4 T cells. Primed WC1<jats:sup>+<\/jats:sup> \u03b3\u03b4 T cells circulated as CD62L<jats:sup>hi<\/jats:sup>\/CD45RO<jats:sup>int<\/jats:sup>\/CD44<jats:sup>lo<\/jats:sup>, characteristics of T<jats:sub>CM<\/jats:sub> cells. When stimulated with antigen, they decreased CD62L, increased CD44 and CD25, and had no change in CD45RO expression. These changes paralleled those of the leptospira antigen\u2010responsive CD4 T cells but differed from those of \u03b3\u03b4 T cells proliferating to mitogen stimulation. This system for <jats:italic>in vivo<\/jats:italic> \u03b3\u03b4 T cell priming is unique, since it relies on a killed antigen to induce memory and may be pertinent to designing vaccines that require type 1 pro\u2010inflammatory cytokines.<\/jats:p>","DOI":"10.1002\/eji.200636216","type":"journal-article","created":{"date-parts":[[2007,4,11]],"date-time":"2007-04-11T16:23:20Z","timestamp":1176308600000},"page":"1204-1216","source":"Crossref","is-referenced-by-count":65,"title":["WC1<sup>+<\/sup> \u03b3\u03b4 T cell memory population is induced by killed bacterial vaccine"],"prefix":"10.1002","volume":"37","author":[{"given":"Seth\u2004L.","family":"Blumerman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn\u2004T.\u2004A.","family":"Herzig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia\u2004L.","family":"Baldwin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,4,19]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(03)00032-2"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.3.1447"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(01)00192-3"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.20.100101.151926"},{"key":"e_1_2_7_5_2","doi-asserted-by":"crossref","first-page":"4628","DOI":"10.1128\/iai.63.12.4628-4633.1995","article-title":"The antituberculous Mycobacterium bovis BCG vaccine is an attenuated mycobacterial producer of phosphorylated nonpeptidic antigens for human \u03b3\u03b4 T cells.","volume":"63","author":"Constant P.","year":"1995","journal-title":"Infect. 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