{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T10:35:19Z","timestamp":1773916519956,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,3,13]],"date-time":"2007-03-13T00:00:00Z","timestamp":1173744000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The proteasome is critically involved in the production of MHC class\u2004I\u2010restricted T cell epitopes. Approximately 20% of all peptides generated by the proteasome are too large for direct presentation by MHC class\u2004I molecules. Reits <jats:italic>et al.<\/jats:italic> (<jats:italic>Immunity<\/jats:italic> 2004. <jats:bold>20:<\/jats:bold> 495\u2013506) suggested that a major portion of proteasomal products are larger than 15\u2004amino acids and require further degradation by the tripeptidyl peptidase\u2004II (TPPII) before becoming ligands of MHC class\u2004I molecules. Using the well\u2010characterized lymphocytic choriomeningitis virus (LCMV) model, the role of TPPII in the processing of several LCMV\u2010derived T cell epitopes was investigated. In contrast to Reits\u2019 proposal, TPPII inhibition and TPPII overexpression experiments revealed that five out of six LCMV\u2010derived CD8<jats:sup>+<\/jats:sup> T cell epitopes were not affected by inhibition of TPPII, while one epitope (GP276) was slightly reduced upon TPPII overexpression. Additionally, we demonstrated that the processing of two epitopes derived from ovalbumin and murine cytomegalovirus were not altered by TPPII inhibition. We propose that TPPII is not generally required for the production of MHC class\u2004I peptides, but the presentation of some peptides can be negatively affected by TPPII.<\/jats:p>","DOI":"10.1002\/eji.200636372","type":"journal-article","created":{"date-parts":[[2007,3,13]],"date-time":"2007-03-13T14:36:46Z","timestamp":1173796606000},"page":"896-904","source":"Crossref","is-referenced-by-count":28,"title":["No essential role for tripeptidyl peptidase\u2004II for the processing of LCMV\u2010derived T cell epitopes"],"prefix":"10.1002","volume":"37","author":[{"given":"Michael","family":"Basler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcus","family":"Groettrup","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,3,28]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.4.1982"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.30.18734"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/80852"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105517200"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00058-X"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5404.978"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni905"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(04)00074-3"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni860"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni859"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1208"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.6.3363"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504569102"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.8.4161"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.9.4801"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.4.2249"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.12.6147"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-5890(98)00053-4"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/381166a0"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-2085"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.8.5440"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.3.1434"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/33443"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.180328897"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1090"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.166.3.668"},{"key":"e_1_2_7_27_2","doi-asserted-by":"crossref","first-page":"8497","DOI":"10.1128\/jvi.71.11.8497-8503.1997","article-title":"DNA immunization: Ubiquitination of a viral protein enhances cytotoxic T\u2010lymphocyte induction and antiviral protection but abrogates antibody induction.","volume":"71","author":"Rodriguez F.","year":"1997","journal-title":"J. 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