{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T22:11:27Z","timestamp":1781561487939,"version":"3.54.5"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,1,11]],"date-time":"2007-01-11T00:00:00Z","timestamp":1168473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Amebiasis is a human disease caused by the protozoan intestinal parasite <jats:italic>Entamoeba histolytica<\/jats:italic>. Vaccine development has focused on the parasite's surface galactose\u2010<jats:italic>N<\/jats:italic>\u2010acetyl\u2010<jats:sc>d\u2010<\/jats:sc>galactosamine inhibitable lectin (Gal\u2010lectin) as a protective antigen. The Gal\u2010lectin is immunogenic and has been shown to induce Th1 cytokines <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo.<\/jats:italic> The immunological basis of the protective immune response elicited by the Gal\u2010lectin is unknown. In this study, we investigated the response of BALB\/c bone marrow\u2010derived DC to <jats:italic>E. histolytica<\/jats:italic> Gal\u2010lectin. Incubation of immature DC with Gal\u2010lectin resulted in activation and maturation after 24\u2004h. FACS analysis demonstrated an up\u2010regulation of DC maturation markers CD80, CD86, CD40 and MHC class II upon exposure to Gal\u2010lectin. The Gal\u2010lectin also induced DC production of IL\u201012, indicating a Th1 response. Gal\u2010lectin\u2010activated DC were able to stimulate T cell proliferation in an allogeneic mixed leukocyte reaction and adoptive transfer of Gal\u2010lectin\u2010treated DC into na\u00efve mice resulted in IFN\u2010\u03b3\u2010producing Gal\u2010lectin\u2010sensitized T cells. The activation of DC by Gal\u2010lectin was mediated by MAPK and NF\u2010\u03baB. These findings indicate that <jats:italic>E. histolytica<\/jats:italic> Gal\u2010lectin is a potent vaccine antigen capable of directly initiating DC maturation and activation characterized by Th1 cytokine production.<\/jats:p>","DOI":"10.1002\/eji.200636476","type":"journal-article","created":{"date-parts":[[2007,1,11]],"date-time":"2007-01-11T15:18:14Z","timestamp":1168528694000},"page":"385-394","source":"Crossref","is-referenced-by-count":36,"title":["Activation of dendritic cells by the Gal\u2010lectin of <i>Entamoeba histolytica<\/i> drives Th1 responses <i>in vitro<\/i> and <i>in vivo<\/i>"],"prefix":"10.1002","volume":"37","author":[{"given":"Catherine\u2004P.\u2004A.","family":"Ivory","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kris","family":"Chadee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,2]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1086\/520355"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.1.400-402.2000"},{"key":"e_1_2_7_3_2","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1128\/iai.57.6.1750-1756.1989","article-title":"Cytokine activation of murine macrophages for in vitro killing of Entamoeba histolytica trophozoites.","volume":"57","author":"Denis M.","year":"1989","journal-title":"Infect. 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