{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:38:43Z","timestamp":1791121123070,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2006,11,16]],"date-time":"2006-11-16T00:00:00Z","timestamp":1163635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The human NKG2D killer lectin\u2010like receptor (KLR) is coupled by the DAP10 adapter to phosphoinositide 3\u2010kinase (PI3\u2004K) and specifically interacts with different stress\u2010inducible molecules (\n                    <jats:italic>i.e.<\/jats:italic>\n                    MICA, MICB, ULBP) displayed by some tumour and virus\u2010infected cells. This KLR is commonly expressed by human NK cells as well as TCR\u03b3\u03b4\n                    <jats:sup>+<\/jats:sup>\n                    and TCR\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T lymphocytes, but it has been also detected in CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells from rheumatoid arthritis and cancer patients. In the present study, we analysed NKG2D expression in human cytomegalovirus (HCMV)\u2010specific CD4\n                    <jats:sup>+<\/jats:sup>\n                    T lymphocytes.\n                    <jats:italic>In vitro<\/jats:italic>\n                    stimulation of peripheral blood mononuclear cells (PBMC) from healthy seropositive individuals with HCMV promoted variable expansion of CD4\n                    <jats:sup>+<\/jats:sup>\n                    NKG2D\n                    <jats:sup>+<\/jats:sup>\n                    T lymphocytes that coexpressed perforin. NKG2D was detected in CD28\n                    <jats:sup>\u2013<\/jats:sup>\n                    and CD28\n                    <jats:sup>dull<\/jats:sup>\n                    subsets and was not systematically associated with the expression of other NK cell receptors (\n                    <jats:italic>i.e.<\/jats:italic>\n                    KIR, CD94\/NKG2 and ILT2). Engagement of NKG2D with specific mAb synergized with TCR\u2010dependent activation of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells, triggering proliferation and cytokine production (\n                    <jats:italic>i.e.<\/jats:italic>\n                    IFN\u2010\u03b3 and TNF\u2010\u03b1). Altogether, the data support the notion that NKG2D functions as a prototypic costimulatory receptor in a subset of HCMV\u2010specific CD4\n                    <jats:sup>+<\/jats:sup>\n                    T lymphocytes and thus may have a role in the response against infected HLA class II\n                    <jats:sup>+<\/jats:sup>\n                    cells displaying NKG2D ligands.\n                  <\/jats:p>","DOI":"10.1002\/eji.200636682","type":"journal-article","created":{"date-parts":[[2006,11,17]],"date-time":"2006-11-17T08:40:50Z","timestamp":1163752850000},"page":"3198-3206","source":"Crossref","is-referenced-by-count":93,"title":["Expression and function of NKG2D in CD4\n                    <sup>+<\/sup>\n                    T cells specific for human 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