{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:46:38Z","timestamp":1777596398887,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,4,11]],"date-time":"2007-04-11T00:00:00Z","timestamp":1176249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Identification and characterization of IgE\u2010inducing antigens are important for elucidating the mechanisms involved in IgE\u2010mediated immune responses in allergic diseases and parasite infections. While many allergens have been characterized, little is known about parasite antigens inducing specific IgE following infection. In order to identify antigens from the nematode\n                    <jats:italic>Nippostrongylus brasiliensis<\/jats:italic>\n                    , we generated an IgE\u2010producing B cell hybridoma from\n                    <jats:italic>N.<\/jats:italic>\n                    \u2004\n                    <jats:italic>brasiliensis<\/jats:italic>\n                    \u2010infected C57BL\/6 mice and constructed a cDNA phage display library from\n                    <jats:italic>N.<\/jats:italic>\n                    \u2004\n                    <jats:italic>brasiliensis<\/jats:italic>\n                    . We successfully cloned and expressed an\n                    <jats:italic>N.<\/jats:italic>\n                    \u2004\n                    <jats:italic>brasiliensis<\/jats:italic>\n                    antigen (Nb\u2010Ag1) that showed specific binding to anti\u2010\n                    <jats:italic>N.<\/jats:italic>\n                    \u2004\n                    <jats:italic>brasiliensis<\/jats:italic>\n                    IgE. Nb\u2010Ag1 localized to the pharynx of adult\n                    <jats:italic>N.<\/jats:italic>\n                    \u2004\n                    <jats:italic>brasiliensis<\/jats:italic>\n                    , suggesting that Nb\u2010Ag1 is a potential pharyngeal gland antigen. Nb\u2010Ag1\u2010specific IgE could be detected in the serum of\n                    <jats:italic>N.<\/jats:italic>\n                    \u2004\n                    <jats:italic>brasiliensis<\/jats:italic>\n                    \u2010infected mice, but only for a short time and only following a challenge infection. In contrast, local administration of Nb\u2010Ag1 during primary, secondary and tertiary infections induced Nb\u2010Ag1\u2010specific IgE\u2010mediated active cutaneous anaphylaxis. Therefore, amongst the high amounts of polyclonal total IgE, low levels of parasite\u2010specific IgE responses are induced during primary helminth infections. Here, we show that even such low levels of parasite\u2010specific IgE are sufficient to prime mast cells\n                    <jats:italic>in vivo<\/jats:italic>\n                    and mediate degranulation.\n                  <\/jats:p>\n                  <jats:p>\n                    See accompanying commentary:\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1002\/eji.200737314\">http:\/\/dx.doi.org\/10.1002\/eji.200737314<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1002\/eji.200737135","type":"journal-article","created":{"date-parts":[[2007,4,11]],"date-time":"2007-04-11T12:26:00Z","timestamp":1176294360000},"page":"1275-1284","source":"Crossref","is-referenced-by-count":24,"title":["Identification and characterization of a novel antigen from the nematode\n                    <i>Nippostrongylus brasiliensis<\/i>\n                    recognized by specific IgE"],"prefix":"10.1002","volume":"37","author":[{"given":"Veronika","family":"Pochanke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Koller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00e9gine","family":"Dayer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Hatak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Burkhard","family":"Ludewig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf\u2004M.","family":"Zinkernagel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Hengartner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathy\u2004D.","family":"McCoy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,4,19]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652001000500011"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.2.1139"},{"key":"e_1_2_7_3_2","doi-asserted-by":"crossref","first-page":"294","DOI":"10.4049\/jimmunol.158.1.294","article-title":"Mice with a targeted deletion of the IgE gene have increased worm burdens and reduced granulomatous inflammation following primary infection with Schistosoma mansoni.","volume":"158","author":"King C. 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