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Analysis of influenza sequences from animal and human isolates using CLUSTALW and a novel proprietary epitope prediction algorithm identified six conserved T cell\u2010reactive regions in several proteins. Immunisation of transgenic mice with a preparation of these six regions as chemically synthesised peptides (FLU\u2010v) induced a specific HLA\u2010A*0201\u2010mediated CD8<jats:sup>+<\/jats:sup> T cell response. This T cell population also reacted against human cells infected with three non\u2010related influenza strains, confirming that the identified regions contain epitopes naturally presented by infected human cells and conserved amongst non\u2010related viruses. Moreover, FLU\u2010v immunisation significantly increased survival of transgenic mice against lethal challenge with influenza. Overall, FLU\u2010v represents a promising influenza vaccine candidate, obviating the need for yearly vaccinations and allowing the stockpiling and initiation of a worldwide vaccination program in advance of a pandemic outbreak.<\/jats:p>","DOI":"10.1002\/eji.200737254","type":"journal-article","created":{"date-parts":[[2007,8,2]],"date-time":"2007-08-02T09:49:50Z","timestamp":1186048190000},"page":"2441-2449","source":"Crossref","is-referenced-by-count":74,"title":["Synthetic multi\u2010epitope peptides identified <i>in silico<\/i> induce protective immunity against multiple influenza serotypes"],"prefix":"10.1002","volume":"37","author":[{"given":"Gregory\u2004A.","family":"Stoloff","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilson","family":"Caparros\u2010Wanderley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,8,27]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.2165\/00019053-199600093-00014"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/172.2.389"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00071-9"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.175.4.1143"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(03)00067-0"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.9.5332"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.12.2063"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.6.2283"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.1.261"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00408-X"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.13.7052-7060.2004"},{"key":"e_1_2_7_12_2","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.4049\/jimmunol.142.4.1366","article-title":"Cytotoxic T cell responses in HLA\u2010A2.1 transgenic mice. 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