{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:37:04Z","timestamp":1790858224189,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,3,26]],"date-time":"2008-03-26T00:00:00Z","timestamp":1206489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>MHC class\u2004II\u2010mediated antigen presentation by B\u2004lymphocytes or dendritic cells (DC) initiates CD4<jats:sup>+<\/jats:sup> T\u2004lymphocyte activation. In B\u2004lymphocytes, MHC class\u2004II peptide presentation has been characterised by recruitment of MHC class\u2004II, F\u2010actin and lipid rafts to the B cell\u2013T cell immunological synapse. We now show that MHC class\u2004II engagement in B\u2004lymphocytes induced lipid raft\u2010independent Rho and Rac activation and that inhibition of either Rho\u2010GTPase activation or actin polymerisation in the B cell abrogated T cell activation without altering B cell\u2013T cell conjugate formation. Short\u2010hairpin RNA studies excluded a role for the Cdc42 effector Wiskott\u2013Aldrich syndrome protein. In contrast, antigen presentation by DC was Rho\u2010GTPase\u2010independent although actin was recruited to the DC\u2013T cell interaction site. Moreover, actin depolymerisation in the DC significantly increased T cell activation without altering the number of DC\u2013T cell conjugates. Finally we show that stable recruitment of HLA\u2010DR to the site of the immunological synapse is not a uniform observation in DC and demonstrate reduced HLA\u2010DR expression at the site of microtubule organising centre polarization. Therefore although actin accumulates in DC and B\u2004lymphocytes at the immunological synapse with antigen\u2010specific T\u2004lymphocytes, this does not reflect comparable functional roles of their actin cytoskeletons in antigen presentation.<\/jats:p>","DOI":"10.1002\/eji.200737455","type":"journal-article","created":{"date-parts":[[2008,4,7]],"date-time":"2008-04-07T20:48:13Z","timestamp":1207601293000},"page":"1096-1105","source":"Crossref","is-referenced-by-count":9,"title":["Contrasting cytoskeletal regulation of MHC class\u2004II peptide presentation by human B cells or dendritic cells"],"prefix":"10.1002","volume":"38","author":[{"given":"Alix","family":"Delaguillaumie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"Marin\u2010Esteban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niclas","family":"Setterblad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence\u2004Jeanson","family":"Leh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Assier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Gelin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Charron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Galy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuala","family":"Mooney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,4,7]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/77842"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E05-01-0005"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.11.5812"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.291.5508.1537"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/25764"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5425.221"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni741"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.4.2030"},{"key":"e_1_2_8_9_2","doi-asserted-by":"crossref","first-page":"812","DOI":"10.4049\/jimmunol.145.3.812","article-title":"MHC class\u2004II\u2010restricted presentation of native protein antigen by B cells is inhibitable by cycloheximide and brefeldin\u2004A.","volume":"145","author":"\u2010Pierre Y.","year":"1990","journal-title":"J. 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