{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:44:11Z","timestamp":1790811851598,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,3,26]],"date-time":"2008-03-26T00:00:00Z","timestamp":1206489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CD8<jats:sup>hi<\/jats:sup>CD57<jats:sup>+<\/jats:sup> T cells have previously been described as effector memory T cells with minimal expansion capacity and high susceptibility to activation\u2010induced cell death. In contrast, we demonstrate here that CD8<jats:sup>hi<\/jats:sup>CD57<jats:sup>+<\/jats:sup> T cells are capable of rapid expansion using multiple techniques including [<jats:sup>3<\/jats:sup>H]thymidine uptake, flow cytometric bead\u2010based enumeration and standard haemocytometer counting. Previous reports can be explained by marked inhibition of activation\u2010induced expansion and increased 7\u2010amino\u2010actinomycin\u2004D uptake by CD8<jats:sup>hi<\/jats:sup>CD57<jats:sup>+<\/jats:sup> T cells following treatment with CFSE, a dye previously used to measure their proliferation, combined with specific media requirements for the growth of this cell subset. The ability of CD8<jats:sup>hi<\/jats:sup>CD57<jats:sup>+<\/jats:sup> T cells to further differentiate is highlighted by a distinct cytokine profile late after activation that includes the unexpected release of high levels of interleukin\u20045. These data indicate that CD8<jats:sup>hi<\/jats:sup>CD57<jats:sup>+<\/jats:sup> T cells should not be considered as \u201cend\u2010stage\u201d effector T cells incapable of proliferation, but represent a highly differentiated subset capable of rapid division and exhibiting novel functions separate from their previously described cytotoxic and IFN\u2010\u03b3 responses.<\/jats:p>","DOI":"10.1002\/eji.200737687","type":"journal-article","created":{"date-parts":[[2008,3,26]],"date-time":"2008-03-26T14:48:15Z","timestamp":1206542895000},"page":"995-1000","source":"Crossref","is-referenced-by-count":50,"title":["Proliferation and interleukin\u20045 production by CD8<sup>hi<\/sup>CD57<sup>+<\/sup> T cells"],"prefix":"10.1002","volume":"38","author":[{"given":"Lee\u2004K.","family":"Chong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca\u2004J.","family":"Aicheler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sian","family":"Llewellyn\u2010Lacey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Tomasec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Brennan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eddie\u2004C.\u2004Y.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,4,7]]},"reference":[{"key":"e_1_2_7_1_2","first-page":"69","article-title":"The role of CD8+, CD57+ cells in human cytomegalovirus and other viral infections.","volume":"99","author":"Wang E. 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