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Immune responses cross\u2010reactive between different dengue serotypes are important in the pathogenesis of dengue disease but it is not known whether previous infection with one flavivirus might affect the clinical course of subsequent infections with other members of the family. CD4<jats:sup>+<\/jats:sup> T cells have been shown to be important in the production of cytokines in response to dengue infection and can demonstrate significant epitope cross\u2010reactivity. Here, we describe the generation and characterisation of CD4<jats:sup>+<\/jats:sup> T cell clones from a patient experiencing acute dengue infection. These clones were DRB1*15<jats:sup>+<\/jats:sup> and recognised epitope variants not only within other dengue viruses but certain other flaviviruses. This cross\u2010reactivity was dependent upon the presence of a five\u2010amino acid core region, consistent with structural observations of class II MHC binding to TCR demonstrating that only a subset of residues within an epitope bound to a class II molecule are \u201cread out\u201d by the TCR. This capacity of CD4<jats:sup>+<\/jats:sup> T cell clones to recognise a given epitope despite considerable variation between viruses may be of pathological significance, particularly in regions where related viruses co\u2010circulate.<\/jats:p>","DOI":"10.1002\/eji.200737699","type":"journal-article","created":{"date-parts":[[2008,3,26]],"date-time":"2008-03-26T14:48:49Z","timestamp":1206542929000},"page":"1050-1057","source":"Crossref","is-referenced-by-count":24,"title":["Preservation of a critical epitope core region is associated with the high degree of flaviviral cross\u2010reactivity exhibited by a dengue\u2010specific CD4<sup>+<\/sup> T cell clone"],"prefix":"10.1002","volume":"38","author":[{"given":"Edward","family":"Moran","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cameron","family":"Simmons","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen","family":"Vinh\u2004Chau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerstin","family":"Luhn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bridget","family":"Wills","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen\u2004Phuong","family":"Dung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Le\u2004Thi\u2004Thu","family":"Thao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tran\u2004Tinh","family":"Hien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy","family":"Farrar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Rowland\u2010Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,4,7]]},"reference":[{"key":"e_1_2_8_1_2","first-page":"311","article-title":"Observations related to pathogenesis of dengue hemorrhagic fever. 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