{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T10:37:51Z","timestamp":1775644671967,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,4,15]],"date-time":"2008-04-15T00:00:00Z","timestamp":1208217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Epstein\u2010Barr virus\u2010induced gene 3 (EBI3) associates with p28 to form IL\u201027 and with IL\u201012p35 to form IL\u201035. IL\u201027R\u03b1<jats:sup>\u2013\/\u2013<\/jats:sup> mice studies indicate that IL\u201027 negatively regulates Th17 cell differentiation. However, no EBI3, p28 or p35\u2010deficiency studies that directly address the role of EBI3, p28 or p35 on Th17 cells have been done. Here, we demonstrate that spleen cells derived from EBI3<jats:sup>\u2013\/\u2013 <\/jats:sup>mice produce significantly higher levels of IL\u201017 as well as IL\u201022 upon stimulation with OVA. <jats:italic>In vitro<\/jats:italic> derived EBI3<jats:sup>\u2013\/\u2013 <\/jats:sup>Th17 cells also produced significantly higher levels of IL\u201017 and IL\u201022 than WT cells. The frequency of IL\u201017\u2010producing cells was also elevated when EBI3<jats:sup>\u2013\/\u2013 <\/jats:sup>cells were cultured under Th17 conditions. In addition, spleen cells from EBI3<jats:sup>\u2013\/\u2013<\/jats:sup> mice immunized with <jats:italic>Listeria monocytogenes<\/jats:italic> produced significantly elevated levels of IL\u201017 and IL\u201022. Furthermore, the Th17 transcription factor ROR\u03b3t was significantly enhanced in EBI3<jats:sup>\u2013\/\u2013 <\/jats:sup>cells. Finally, EBI3<jats:sup>\u2013\/\u2013 <\/jats:sup>mice exhibited a reduced bacterial load following an acute challenge with <jats:italic>L. monocytogenes <\/jats:italic>or a re\u2010challenge of previously immunized mice, suggesting that EBI3 negatively regulates both innate and adaptive immunity. Taken together, these data provide direct evidence that EBI3 negatively regulates the expression of IL\u201017, IL\u201022 and ROR\u03b3t as well as protective immunity against <jats:italic>L. monocytogenes<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/eji.200838145","type":"journal-article","created":{"date-parts":[[2008,4,15]],"date-time":"2008-04-15T17:49:49Z","timestamp":1208281789000},"page":"1204-1214","source":"Crossref","is-referenced-by-count":88,"title":["Epstein\u2010Barr virus\u2010induced gene 3 negatively regulates IL\u201017, IL\u201022 and 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