{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T03:56:58Z","timestamp":1790308618582,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2008,9,29]],"date-time":"2008-09-29T00:00:00Z","timestamp":1222646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We determined the efficacy of <jats:italic>in vitro<\/jats:italic> expanded P14 TCR transgenic CD8 T cells to mediate tumor cell elimination and to protect against viral infection in mice. Contrary to previous studies, an adoptive transfer model without lymphodepletion, vaccination or cytokine treatment was used. Antigen\u2010activated P14 T cells cultured in IL\u20102\u2010containing medium for 7 days (P14<jats:sub>IL\u20102<\/jats:sub>) exhibited potent effector cell functions <jats:italic>in vitro<\/jats:italic> but did not confer protection against melanoma growth or viral infection. In contrast, P14 T cells cultured in IL\u201015 (P14<jats:sub>IL\u201015<\/jats:sub>) were highly effective <jats:italic>in vivo<\/jats:italic> although they displayed only moderate effector functions <jats:italic>in vitro<\/jats:italic>. Therapeutic efficacy correlated with the survival of the transferred T cells in the recipients: P14<jats:sub>IL\u20102<\/jats:sub> cells disappeared rapidly whereas P14<jats:sub>IL\u201015<\/jats:sub> cells persisted for prolonged time. Decreasing the IL\u20102 concentration in the culture media improved <jats:italic>in vivo<\/jats:italic> survival and efficacy but also lowered the cell yield of the cultures. Finally, we could extend the findings with monoclonal P14 T cells to polyclonal CD8 T cells. Thus, <jats:italic>in vitro<\/jats:italic> expansion of antigen\u2010specific CD8 T cells in IL\u201015 allowed the generation of substantial numbers of T cells without inducing terminally differentiated effector cells that turned out to be unfavorable in the transfer model examined here.<\/jats:p>","DOI":"10.1002\/eji.200838426","type":"journal-article","created":{"date-parts":[[2008,9,29]],"date-time":"2008-09-29T09:22:22Z","timestamp":1222680142000},"page":"2874-2885","source":"Crossref","is-referenced-by-count":40,"title":["Efficacy of IL\u20102\u2010 <i>versus<\/i> IL\u201015\u2010stimulated CD8 T cells in adoptive immunotherapy"],"prefix":"10.1002","volume":"38","author":[{"given":"Katja","family":"Mueller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Schweier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanspeter","family":"Pircher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,10,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1100369"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI31446"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2006.00391.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.25.022106.141527"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1901"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI13296"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-12-4827"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24480"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307298101"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503726102"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19861215)58:12<2764::AID-CNCR2820581235>3.0.CO;2-Z"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.7.953"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.12.7315"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.08.007"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.22.012703.104702"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.12.7125"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200009)30:9<2507::AID-IMMU2507>3.0.CO;2-V"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm827"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5466.675"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/83253"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3523754"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00429-6"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.57.5.763"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80664-0"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20041389"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610115104"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-08-2814"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0216356"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.03.006"},{"key":"e_1_2_6_31_2","doi-asserted-by":"crossref","first-page":"698","DOI":"10.4049\/jimmunol.136.2.698","article-title":"Biology of cloned cytotoxic T lymphocytes specific for lymphocytic choriomeningitis virus. VI. Migration and activity in vivo in acute and persistent infection","volume":"136","author":"Byrne J. A.","year":"1986","journal-title":"J. Immunol."},{"key":"e_1_2_6_32_2","doi-asserted-by":"crossref","first-page":"507","DOI":"10.4049\/jimmunol.163.1.507","article-title":"Efficient transfer of a tumor antigen\u2010reactive TCR to human peripheral blood lymphocytes confers anti\u2010tumor reactivity","volume":"163","author":"Clay T. M.","year":"1999","journal-title":"J. Immunol."},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1001-957"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1129003"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830230834"},{"key":"e_1_2_6_36_2","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.4049\/jimmunol.161.5.2187","article-title":"Tumor\u2010infiltrating lymphocytes exhibiting high ex vivo cytolytic activity fail to prevent murine melanoma tumor growth in vivo","volume":"161","author":"Prevost\u2010Blondel A.","year":"1998","journal-title":"J. Immunol."},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(91)90018-U"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.1.195"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.200838426","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.200838426","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T19:11:40Z","timestamp":1695928300000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.200838426"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10]]},"references-count":37,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2008,10]]}},"alternative-id":["10.1002\/eji.200838426"],"URL":"https:\/\/doi.org\/10.1002\/eji.200838426","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10]]}}}