{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:20:19Z","timestamp":1787505619896,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Psoriasis vulgaris is an autoimmune dermatosis with Th17 infiltration. Prolactin (PRL) may participate in the pathogenesis of psoriasis. The chemokine CCL20 recruits Th17 cells, and CCL20 production by epidermal keratinocytes is enhanced in psoriatic lesions. We examined the\n                    <jats:italic>in vitro<\/jats:italic>\n                    effects of PRL on CCL20 production in human keratinocytes. PRL increased basal and IL\u201017\u2010induced CCL20 secretion, and mRNA expression in keratinocytes. CCL20 production by PRL was suppressed by antisense oligonucleotides against the AP\u20101 components c\u2010Fos and c\u2010Jun, whereas that by IL\u201017 was suppressed by antisense NF\u2010\u03baB p50 and p65. CCL20 production induced by PRL plus IL\u201017 was suppressed by antisense c\u2010Fos, c\u2010Jun, p50, and p65. PRL alone increased the transcriptional activity of AP\u20101, and c\u2010Fos and c\u2010Jun expression; moderately enhanced NF\u2010\u03baB activity and I\u03baB\u03b1 phosphorylation; and potently increased IL\u201017\u2010induced NF\u2010\u03baB activity. MEK and JNK inhibitors suppressed PRL\u2010 or PRL\u2010plus\u2010IL\u201017\u2010induced CCL20 production and AP\u20101 activities. MEK inhibitor suppressed PRL\u2010induced c\u2010Fos expression, whereas JNK inhibitor suppressed c\u2010Jun expression. PRL induced ERK and JNK phosphorylation. These results suggest that PRL may enhance basal and IL\u201017\u2010induced CCL20 production in keratinocytes by AP\u20101 and NF\u2010\u03baB activation, which is partially mediated\n                    <jats:italic>via<\/jats:italic>\n                    MEK\/ERK and JNK. PRL may promote Th17 infiltration into psoriatic lesions\n                    <jats:italic>via<\/jats:italic>\n                    CCL20.\n                  <\/jats:p>","DOI":"10.1002\/eji.200838852","type":"journal-article","created":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T07:35:22Z","timestamp":1239003322000},"page":"996-1006","source":"Crossref","is-referenced-by-count":40,"title":["Prolactin enhances basal and IL\u201017\u2010induced CCL20 production by human keratinocytes"],"prefix":"10.1002","volume":"39","author":[{"given":"Naoko","family":"Kanda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayaka","family":"Shibata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yayoi","family":"Tada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyoko","family":"Nashiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunihiko","family":"Tamaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.114707"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2002.120568"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5701213"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.11.7423"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1496"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1497"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20080397"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.12.6621"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.10.6676"},{"key":"e_1_2_6_11_2","first-page":"639","article-title":"Extrapituitary prolactin: distribution, regulation, functions, and clinical aspects","volume":"17","author":"Ben\u2010Jonathan N.","year":"1996","journal-title":"Endocr. Rev."},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.131.6.3019"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10911-008-9072-x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0514"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11102-005-5082-5"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1159\/000017981"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2000.03792.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1177\/37.5.2703700"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.050468"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12348944"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12365203"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-1639"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb05415.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208241200"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.09.024"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00293.2007"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240420403"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.49.31496"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgh198"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.6.4064"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxm145"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-0343"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.8.2749-2761.2003"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00306.2006"},{"key":"e_1_2_6_35_2","first-page":"111","article-title":"X\u2010linked inhibitor of apoptosis protein regulates human interleukin\u20106 in umbilical vein endothelial cells via stimulation of the nuclear factor\u2010\u03baB and MAP kinase signaling pathways","volume":"58","author":"Resch U.","year":"2006","journal-title":"Pharmacol. Rev."},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.04250.x"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601020"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1210\/rp.57.1.435"},{"key":"e_1_2_6_39_2","first-page":"1139","article-title":"Involvement of SHC, GRB2, SOS and RAS in prolactin signal transduction in mammary epithelial cells","volume":"13","author":"Das R.","year":"1996","journal-title":"Oncogene"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1210\/me.2002-0422"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.25.15719"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02338.x"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.9.4767"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306976200"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.10.6504"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C600256200"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0611589104"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.04.069"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20714"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi034217s"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0906-6705.2003.00086.x"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65283-2"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.200838852","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.200838852","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T00:28:08Z","timestamp":1695947288000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.200838852"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,4]]},"references-count":51,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,4]]}},"alternative-id":["10.1002\/eji.200838852"],"URL":"https:\/\/doi.org\/10.1002\/eji.200838852","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,4]]}}}