{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T12:29:42Z","timestamp":1772454582165,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Swiss National Foundation","award":["632-066020"],"award-info":[{"award-number":["632-066020"]}]},{"DOI":"10.13039\/501100004201","name":"Oncosuisse","doi-asserted-by":"crossref","award":["01312-02-2003"],"award-info":[{"award-number":["01312-02-2003"]}],"id":[{"id":"10.13039\/501100004201","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Cancer League of the Canton of Bern"},{"name":"Switzerland and the Roche Research Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CTL are induced by two pathways, <jats:italic>i.e.<\/jats:italic> direct priming, where tumor cells present tumor antigens to na\u00efve specific CTL, and cross\u2010priming, where professional APC cross\u2010present captured tumor antigens to CTL. Here, we examined direct priming <jats:italic>versus<\/jats:italic> cross\u2010priming after immunizing (H\u20102<jats:sup>b<\/jats:sup>\u00d7H\u20102<jats:sup>d<\/jats:sup>) F1 mice with either H\u20102<jats:sup>b<\/jats:sup> or H\u20102<jats:sup>d<\/jats:sup> positive tumor cells transfected with the GP or nucleoprotein (NP) of lymphocytic choriomeningitis virus (LCMV). Cross\u2010priming was observed for the immunodominant epitopes LCMV\u2010gp33 and \u2010np118, although direct induction resulted in higher CTL frequencies. In contrast, CTL specific for the subdominant epitopes LCMV\u2010gp283 or \u2010np396 were induced only if epitopes were presented directly on MHC class I molecules of the immunizing cell. The broader repertoire and the higher CTL frequencies induced after vaccination with haplotype\u2010matched tumor cells resulted in more efficient anti\u2010tumor and antiviral protection. Firstly, our results indicate that certain virus and tumor antigens may not be detected by CD8<jats:sup>+<\/jats:sup> T cells because of impaired cross\u2010priming. Secondly, efficient cross\u2010priming contributes to the immunodominant nature of a tumor\u2010specific CTL epitope. Thirdly, vaccine strategies using autologous or syngenic antigen\u2010expressing cells induce a broader repertoire of tumor\u2010specific CTL and higher CTL frequencies.<\/jats:p>","DOI":"10.1002\/eji.200838901","type":"journal-article","created":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T12:26:07Z","timestamp":1233577567000},"page":"704-716","source":"Crossref","is-referenced-by-count":17,"title":["CTL induction by cross\u2010priming is restricted to immunodominant epitopes"],"prefix":"10.1002","volume":"39","author":[{"given":"Viktor","family":"Pavelic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias S.","family":"Matter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Mumprecht","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Breyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian F.","family":"Ochsenbein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,3,12]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(05)80059-5"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/35082583"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910620305"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7761853"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03290"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1516"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1096268"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308345101"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1096378"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0501953102"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/32183"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020161"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2004.00142.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.4.2244"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200208)32:8<2356::AID-IMMU2356>3.0.CO;2-Z"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.11.6753"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.082107699"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.cji.0000175496.51594.8b"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-08-3105"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.7.3577"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-4372"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.2.1061-1064.2001"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-004-0560-6"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20001021"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.6.3095"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.5.763"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.8.4222"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/2001"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7513904"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.4.1070"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324257"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.5.1615"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.10.5415"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.2.796"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.2.700"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(02)00009-2"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00081-5"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00480-6"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.2.409"},{"key":"e_1_2_6_41_2","doi-asserted-by":"crossref","first-page":"723","DOI":"10.4049\/jimmunol.163.2.723","article-title":"Antigen concentration and precursor frequency determine the rate of CD8+T cell tolerance to peripherally expressed antigens","volume":"163","author":"Morgan D. 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