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It has been suggested that the functional maintenance of T\u2010cell memory requires the expression of Sca1 on a specialized population of memory T cells termed \u201cmemory stem cells\u201d. Here, we evaluate the requirement for Sca1 in the primary T\u2010cell response to virus infection, and in the establishment and maintenance of T\u2010cell memory. We find that Sca1 expression increases on almost all CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells during virus infection, and remains high on virus\u2010specific memory cells. However, Sca1\u2010deficient (Sca1KO) mice generate normal primary T\u2010cell responses to infection; the kinetics, the immunodominance hierarchy, and the absolute numbers of CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells are essentially indistinguishable from those observed in WT mice. Furthermore, by several criteria, primary and memory T cells in Sca1\u2010deficient mice are phenotypically and functionally normal. These data indicate that Sca1, although perhaps a useful marker of virus\u2010specific memory T cells, is not required for the regulation of T\u2010cell quantity or quality, or for the development of a competent pool of memory cells.<\/jats:p>","DOI":"10.1002\/eji.200838959","type":"journal-article","created":{"date-parts":[[2009,4,21]],"date-time":"2009-04-21T09:20:45Z","timestamp":1240305645000},"page":"1494-1504","source":"Crossref","is-referenced-by-count":24,"title":["Mice deficient in stem cell antigen\u20101 (Sca1, Ly\u20106A\/E) develop normal primary and memory CD4<sup>+<\/sup> and CD8<sup>+<\/sup> T\u2010cell responses to virus infection"],"prefix":"10.1002","volume":"39","author":[{"given":"Jason K.","family":"Whitmire","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boreth","family":"Eam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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