{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:56:24Z","timestamp":1791035784855,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2010,2,5]],"date-time":"2010-02-05T00:00:00Z","timestamp":1265328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>NK cells are important mediators of the early defense. In mice, immature and mature NK (mNK) cells constitutively express the TNF receptor family member CD27; however, mNK cells eventually lose CD27 expression and become resting NK cells. Interaction of CD27 with its ligand, CD70, enhances proliferation and effector functions of NK cells. We used mice that constitutively express CD70 on B cells (CD70\u2010Tg) to study the <jats:italic>in vivo<\/jats:italic> effects of continuous triggering of CD27 on NK cells. Continuous CD70\u2010CD27 interaction resulted in strongly down\u2010modulated CD27 expression on NK cells and gradually reduced absolute NK cell numbers. This reduction was most prominent in the mNK cell subpopulation and was at least partially due to increased apoptosis. Residual NK cells showed lower expression of activating Ly49 receptors and normal (liver) or decreased (spleen) IFN\u2010\u03b3 production. Nevertheless, NK cells from CD70\u2010Tg mice displayed higher YAC\u20101 killing capacities. CD70\u2010Tg NK cells exhibited up\u2010regulated expression of NKG2D, which is in accordance with the increased YAC\u20101 lysis, as this is mainly NKG2D\u2010dependent. Taken together, this study is the first to demonstrate that continuous CD70 triggering of CD27 on NK cells <jats:italic>in vivo<\/jats:italic> results in a severe reduction of NK cells. On a single cell basis, however, residual NK cells display enhanced cytotoxicity.<\/jats:p>","DOI":"10.1002\/eji.200939251","type":"journal-article","created":{"date-parts":[[2010,2,5]],"date-time":"2010-02-05T14:09:17Z","timestamp":1265378957000},"page":"1107-1117","source":"Crossref","is-referenced-by-count":26,"title":["Continuous CD27 triggering <i>in vivo<\/i> strongly reduces NK cell numbers"],"prefix":"10.1002","volume":"40","author":[{"given":"Veerle","family":"De Colvenaer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Taveirne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Hamann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex M.","family":"de Bruin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda","family":"De Smedt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Taghon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart","family":"Vandekerckhove","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Plum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene","family":"van Lier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges","family":"Leclercq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,4,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni0102-6"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1114"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/369031a0"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.12.5679"},{"key":"e_1_2_6_6_2","doi-asserted-by":"crossref","first-page":"4248","DOI":"10.1182\/blood.V92.11.4248","article-title":"Role of spontaneous and interleukin\u20102\u2010induced natural killer cell activity in the cytotoxicity and rejection of Fas(+) and Fas(\u2212) tumor cells","volume":"92","author":"Bradley M.","year":"1998","journal-title":"Blood"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.4.2068"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-08-3262"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.5.2391"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1998.1252"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.7.3667"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.4.1763"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115526"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80453-5"},{"key":"e_1_2_6_15_2","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.4049\/jimmunol.154.4.1653","article-title":"Transplantable NK cell progenitors in murine bone\u2010marrow","volume":"154","author":"Moore T.","year":"1995","journal-title":"J. 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