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To assess the role of Fyn for antigen\u2010driven negative selection and Treg development, which are both dependent on the strength and nature of TCR signaling, we generated mice that co\u2010express the transgenes for OVA and the OT\u2010II TCR, which recognizes a peptide from OVA. In mice expressing both transgenes, negative selection, Treg development in the thymus, and the number of Treg in the periphery were each unaffected by ablation of Fyn. Moreover, <jats:italic>fyn<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> Treg were functional, as assessed <jats:italic>in vitro<\/jats:italic>. We further tested the role of Fyn for the adaptor function of c\u2010Cbl, using mice containing a point mutation in c\u2010Cbl that abolishes its E3 ubiquitin ligase function but maintains its adaptor function. The functional and signaling properties of this mutant c\u2010Cbl were unaltered in <jats:italic>fyn<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> thymocytes. Combined, these data indicate that Fyn was not required for the induction of central tolerance by negative selection, the adaptor protein role of c\u2010Cbl, or the normal development and function of Treg.<\/jats:p>","DOI":"10.1002\/eji.200939645","type":"journal-article","created":{"date-parts":[[2009,11,10]],"date-time":"2009-11-10T18:37:15Z","timestamp":1257878235000},"page":"539-547","source":"Crossref","is-referenced-by-count":2,"title":["Unaltered negative selection and Treg development of self\u2010reactive thymocytes in TCR transgenic Fyn\u2010deficient mice"],"prefix":"10.1002","volume":"40","author":[{"given":"Alusha A.","family":"Mamchak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine B. 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