{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T17:06:18Z","timestamp":1761843978508},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,2,23]],"date-time":"2010-02-23T00:00:00Z","timestamp":1266883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Most novel vaccines against infectious diseases are based on recombinant Ag; however, only few studies have compared Ag\u2010specific immune responses induced by natural infection with that induced by the same Ag in a recombinant form. Here, we studied the epitope recognition pattern of the tuberculosis vaccine Ag, TB10.4, in a recombinant form, or when expressed by the pathogen <jats:italic>Mycobacterium tuberculosis (M.tb)<\/jats:italic>, or by the current anti\u2010tuberculosis vaccine, Mycobacterium bovis BCG. We showed that BCG and <jats:italic>M.tb<\/jats:italic> induced a similar CD4<jats:sup>+<\/jats:sup> T\u2010cell specific TB10.4 epitope\u2010pattern, which differed completely from that induced by recombinant TB10.4. This difference was not due to post\u2010translational modifications of TB10.4 or because TB10.4 is secreted from BCG and <jats:italic>M.tb<\/jats:italic> as a complex with Rv0287. In addition, BCG and TB10.4\/CAF01 were both taken up by DC and macrophages <jats:italic>in vivo<\/jats:italic>, and <jats:italic>in vitro<\/jats:italic> uptake experiments revealed that both TB10.4 and BCG were transported to Lamp<jats:sup>+<\/jats:sup>\u2010compartments. BCG and TB10.4 however, were directed to different types of Lamp<jats:sup>+<\/jats:sup>\u2010compartments in the same APC, which may lead to different epitope recognition patterns. In conclusion, we show that different vectors can induce completely different recognition of the same protein.<\/jats:p>","DOI":"10.1002\/eji.200939830","type":"journal-article","created":{"date-parts":[[2010,2,23]],"date-time":"2010-02-23T17:58:26Z","timestamp":1266947906000},"page":"1342-1354","source":"Crossref","is-referenced-by-count":19,"title":["Difference in TB10.4 T\u2010cell epitope recognition following immunization with recombinant TB10.4, BCG or infection with <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1002","volume":"40","author":[{"given":"Rolf","family":"Billeskov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael V.","family":"Grandal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Poulsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan P.","family":"Christensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Winter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carina","family":"Vingsbo\u2010Lundberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Truc T. 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