{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:14:40Z","timestamp":1790140480109,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Rapid IgE desensitization provides temporary tolerization for patients who have presented severe hypersensitivity reactions to food and drugs, protecting them from anaphylaxis, but the underlying mechanisms are still incompletely understood. Thus, here we develop an effective and reproducible in vitro model of rapid IgE desensitization for mouse BM\u2010derived mast cells (BMMCs) under physiologic calcium conditions, and we characterize its antigen specificity and primary events. BMMCs were challenged with DNP\u2010human serum albumin conjugated (DNP\u2010HSA) and\/or OVA antigens, delivered either as a single dose (activation) or as increasing sequential doses (desensitization). Compared to activated cells, desensitized BMMCs had impaired degranulation, calcium flux, secretion of arachidonic acid products, early and late TNF\u2010\u03b1 production, IL\u20106 production, and phosphorylation of STAT6 and p38 mitogen\u2010activated protein kinase (p38 MAPK). OVA\u2010desensitized cells responded to DNP and DNP\u2010desensitized cells responded to OVA, proving specificity. Internalization of specific antigen, IgE and high\u2010affinity receptor for IgE (Fc\u03b5RI) were impaired in desensitized BMMCs. Our results demonstrate that rapid IgE desensitization is antigen specific and inhibits early and late mast cell activation responses and internalization of the antigen\/IgE\/Fc\u03b5RI complexes.<\/jats:p>","DOI":"10.1002\/eji.201040810","type":"journal-article","created":{"date-parts":[[2011,1,31]],"date-time":"2011-01-31T08:12:41Z","timestamp":1296461561000},"page":"1004-1013","source":"Crossref","is-referenced-by-count":126,"title":["Rapid IgE desensitization is antigen specific and impairs early and late mast cell responses targeting Fc\u03b5RI internalization"],"prefix":"10.1002","volume":"41","author":[{"given":"Maria del Carmen","family":"Sancho\u2010Serra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Simarro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Castells","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,3]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198706253162603"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa070030"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0b013e3280108716"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2005.06.028"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.02.044"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3038.2007.00670.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)60026-9"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2004.08.002"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2072"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00742.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"crossref","first-page":"492","DOI":"10.4049\/jimmunol.135.1.492","article-title":"Biochemical analysis of desensitization of mouse mast cells","volume":"135","author":"Ishizaka T.","year":"1985","journal-title":"J. Immunol."},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1995.tb00033.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1998.tb03768.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.63.1.59"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1159\/000087355"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)61136-2"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v29.i2.40"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.1.421"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.9.4737"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.12.6358"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2008.09.002"},{"key":"e_1_2_6_23_2","doi-asserted-by":"crossref","first-page":"2760","DOI":"10.4049\/jimmunol.151.5.2760","article-title":"Effects of subunit mutation on the localization to coated pits and internalization of cross\u2010linked IgE\u2010receptor complexes","volume":"151","author":"Mao S. Y.","year":"1993","journal-title":"J. Immunol."},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-07-102111"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021806"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.06.026"},{"key":"e_1_2_6_27_2","doi-asserted-by":"crossref","first-page":"501","DOI":"10.4049\/jimmunol.139.2.501","article-title":"Basic characteristics of human lung mast cell desensitization","volume":"139","author":"MacGlashan D.","year":"1987","journal-title":"J. Immunol."},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.04.019"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxm070"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.9.5791"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/87749"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.4.1070"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90434-D"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.1.123"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.201040810","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.201040810","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:44:45Z","timestamp":1697503485000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.201040810"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2011,4]]}},"alternative-id":["10.1002\/eji.201040810"],"URL":"https:\/\/doi.org\/10.1002\/eji.201040810","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,3]]}}}