{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T19:05:06Z","timestamp":1774119906228,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,12,3]],"date-time":"2010-12-03T00:00:00Z","timestamp":1291334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Burkholderia pseudomallei<\/jats:italic>causes melioidosis, a disease with a wide range of possible outcomes, from seroconversion and dormancy to sepsis and death. This spectrum of host\u2013pathogen interactions poses challenging questions about the heterogeneity in immunity to<jats:italic>B. pseudomallei<\/jats:italic>. Models show protection to be dependent on CD4<jats:sup>+<\/jats:sup>cells and IFN\u2010\u03b3, but little is known about specific target antigens. Having previously implicated the ABC transporter, LolC, in protective immunity, we here use epitope prediction, HLA\u2010binding studies, HLA\u2010transgenic models and studies of T cells from seropositive individuals to characterize HLA\u2010restricted LolC responses. Immunized mice showed long\u2010lasting memory to the protein, whereas predictive algorithms identified epitopes within LolC that subsequently demonstrated strong HLA class II binding. Immunization of HLA\u2010DR transgenics with LolC stimulated T\u2010cell responses to four of these epitopes. Furthermore, the responsiveness of HLA transgenics to LolC revealed a hierarchy supportive of HLA polymorphism\u2010determined differential susceptibility. Seropositive human donors of diverse HLA class II types showed T\u2010cell responses to LolC epitopes, which are conserved among<jats:italic>Burkholderia<\/jats:italic>species including<jats:italic>Burkholderia cenocepacia<\/jats:italic>, associated with life\u2010threatening<jats:italic>cepacia<\/jats:italic>complex in cystic fibrosis patients and<jats:italic>Burkholderia mallei<\/jats:italic>, which causes glanders. These findings suggest a role for LolC epitopes in multiepitope vaccine design for melioidosis and related diseases.<\/jats:p>","DOI":"10.1002\/eji.201040881","type":"journal-article","created":{"date-parts":[[2010,11,19]],"date-time":"2010-11-19T12:52:25Z","timestamp":1290171145000},"page":"107-115","source":"Crossref","is-referenced-by-count":8,"title":["CD4<sup>+<\/sup>T\u2010cell immunity to the<i>Burkholderia pseudomallei<\/i>ABC transporter LolC in melioidosis"],"prefix":"10.1002","volume":"41","author":[{"given":"Karen K.","family":"Chu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patcharaporn","family":"Tippayawat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola J.","family":"Walker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah V.","family":"Harding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen S.","family":"Atkins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Maillere","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory J.","family":"Bancroft","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ganjana","family":"Lertmemongkolchai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel M.","family":"Altmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,3]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200204000-00005"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1385"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.383-416.2005"},{"key":"e_1_2_6_5_2","unstructured":"NIAID Biodefense Research Agenda for Category B and C Priority Pathogens Jan2003."},{"key":"e_1_2_6_6_2","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1001\/archinte.1991.00400030135027","article-title":"Melioidosis. 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