{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T03:01:42Z","timestamp":1791255702409,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,12,3]],"date-time":"2010-12-03T00:00:00Z","timestamp":1291334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human V\u03b39V\u03b42 T cells are potent anti\u2010tumor lymphocytes that specifically respond to pyrophosphate (phospho\u2010) antigens, which constitute the basis of current \u03b3\u03b4 T\u2010cell\u2010based immunotherapy strategies. Despite a clear involvement of the TCR, the costimulation requirements of V\u03b39V\u03b42 T cells remain ill\u2010defined. Here, we show that the expression of the CD27 receptor by the vast majority of V\u03b39V\u03b42 peripheral blood lymphocytes endows them with enhanced proliferative capacity upon ligation by its unique ligand CD70, a tumor necrosis factor superfamily member expressed on lymphoma B\u2010cells but also on TCR\u2010activated \u03b3\u03b4 T cells. Moreover, V\u03b39V\u03b42 T\u2010cell treatment with soluble recombinant CD70 induced calcium signals and increased transcription of anti\u2010apoptotic <jats:italic>Bcl2a1<\/jats:italic> and cell\u2010cycle\u2010promoting <jats:italic>Cyclin D2<\/jats:italic> genes. We further demonstrate that the manipulation of CD70\u2013CD27 interactions significantly impacted on V\u03b39V\u03b42 T\u2010cell survival, proliferation and cytokine secretion, in both loss\u2010of\u2010function and gain\u2010of\u2010function experiments. Thus, CD27 coreceptor signals strongly promoted the expansion of Th1\u2010biased, CD27<jats:sup>+<\/jats:sup> V\u03b39V\u03b42 peripheral blood lymphocytes in the context of TCR\u2010mediated stimulation with phosphoantigens. These data collectively establish a novel role for the CD70\u2013CD27 axis in human \u03b3\u03b4 T\u2010cell activation and hence open new perspectives for its modulation in clinical settings.<\/jats:p>","DOI":"10.1002\/eji.201040905","type":"journal-article","created":{"date-parts":[[2010,10,27]],"date-time":"2010-10-27T08:11:35Z","timestamp":1288167095000},"page":"195-201","source":"Crossref","is-referenced-by-count":100,"title":["CD70\u2013CD27 interactions provide survival and proliferative signals that regulate T cell receptor\u2010driven activation of human \u03b3\u03b4 peripheral blood lymphocytes"],"prefix":"10.1002","volume":"41","author":[{"given":"Ana","family":"deBarros","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Chaves\u2010Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"d'Orey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie C.","family":"Ribot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Silva\u2010Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,12,3]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063916"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-3069"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-12-196287"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-08-237123"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-0199"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-12-3665"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.2174\/1566524013363627"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/592971"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2006.00479.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2010.04.004"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-07-101691"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005657"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2003.00034.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2005.04.004"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030235"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1717"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0810059106"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1110267"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-04-1016"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.182.1.718"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.10.6039"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070176"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-11-057216"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.10.009"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-148007"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.201040905","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.201040905","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T04:18:46Z","timestamp":1697516326000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.201040905"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12,3]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["10.1002\/eji.201040905"],"URL":"https:\/\/doi.org\/10.1002\/eji.201040905","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12,3]]}}}