{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:35:04Z","timestamp":1790634904198,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2011,9,22]],"date-time":"2011-09-22T00:00:00Z","timestamp":1316649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The nature of CD4<jats:sup>+<\/jats:sup> T\u2010cell responses after skin immunization and the role of migrating DCs in the presence of adjuvants in the elicited response are interesting issues to be investigated. Here, we evaluated the priming of CD4<jats:sup>+<\/jats:sup> T cells following ear immunization with low doses of model antigens in combination with either cholera toxin (CT) or the non\u2010toxic \u03b2 CT subunit (CTB) as an adjuvant. Following immunization with CT, we found efficient antigen presentation that is reflected in the production of IFN\u2010\u03b3 and IL\u201017 by CD4<jats:sup>+<\/jats:sup> T cells over IL\u20104 or IL\u20105 production. The CTB\u2010induced activation of DCs in the ear occurred without visible inflammation, which reflects a similar type of CD4<jats:sup>+<\/jats:sup> T\u2010cell differentiation. In both cases, the elicited response was dependent on the presence of migrating skin cells. Remarkably, immunization with CT or with CTB led to the induction of a delayed\u2010type hypersensitivity (DTH) response in the ear. The DTH response that was induced by CT immunization was dependent on IL\u201017 and partially dependent on IFN\u2010\u03b3 activity. These results indicate that both CT and CTB induce an efficient CD4<jats:sup>+<\/jats:sup> T\u2010cell response to a co\u2010administered antigen following ear immunization that is dependent on migrating DCs.<\/jats:p>","DOI":"10.1002\/eji.201040997","type":"journal-article","created":{"date-parts":[[2011,7,26]],"date-time":"2011-07-26T14:24:57Z","timestamp":1311690297000},"page":"2894-2904","source":"Crossref","is-referenced-by-count":22,"title":["Intradermal immunization in the ear with cholera toxin and its non\u2010toxic \u03b2 subunit promotes efficient Th1 and Th17 differentiation dependent on migrating DCs"],"prefix":"10.1002","volume":"41","author":[{"given":"David","family":"Meza\u2010S\u00e1nchez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gibr\u00e1n","family":"P\u00e9rez\u2010Montesinos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"S\u00e1nchez\u2010Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura C.","family":"Bonifaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,9,22]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/79734"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00365-5"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1711.2004.01216.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200501071"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2005.10.008"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2005.04.004"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912817107"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021109"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0807126106"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425196"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00506-5"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2000.tb01531.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20091844"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706942104"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(98)01397-8"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.672-679.2000"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.10.5267"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/9.10.1555"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005190"},{"key":"e_1_2_6_21_2","doi-asserted-by":"crossref","unstructured":"Datta S. 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