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However, the generation of effective antiviral or autoreactive adaptive immune responses requires blocking of immunosuppression by Tregs. In this study, we show that TLR7 ligands reduce the number of Tregs generated de novo from na\u00efve murine T cells in vitro and in vivo. In the presence of TLR7\u2010activated splenic DCs, Foxp3 was transiently induced in na\u00efve T cells by TGF\u2010\u03b2 but was downregulated at later time points. Neutralization experiments revealed that loss of Foxp3 after initial induction was mostly dependent on IL\u20106 produced in the DC\u2013T\u2010cell cocultures containing TLR7 ligands. Thus, under the influence of TLR7 ligands fewer Tregs were generated and these expressed lower levels of Foxp3 correlating with a reduced capacity to suppress responder T\u2010cell proliferation. Thus, we provide evidence that TLR7 ligands affect Treg\u2010dependent immune regulation and may thereby contribute to the development of autoimmune diseases such as systemic lupus erythematosus.<\/jats:p>","DOI":"10.1002\/eji.201041014","type":"journal-article","created":{"date-parts":[[2011,3,7]],"date-time":"2011-03-07T14:53:33Z","timestamp":1299509613000},"page":"1334-1343","source":"Crossref","is-referenced-by-count":48,"title":["Activation of dendritic cells via TLR7 reduces Foxp3 expression and suppressive function in induced 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