{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:34:52Z","timestamp":1791153292489,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2011,8,26]],"date-time":"2011-08-26T00:00:00Z","timestamp":1314316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Immunizations via the i.n. and intravaginal (ivag) routes effectively generate strong genital tract antibody\u2010mediated immunity. To what extent the same is true for T\u2010cell responses is incompletely known. Therefore, we set out to investigate optimal conditions for stimulation of genital tract CD4<jats:sup>+<\/jats:sup> T\u2010cell responses, using adoptive transfer of mouse DO11.10 TCR transgenic T cells specific for OVA and OVA conjugated to cholera toxin (CT) as an immunogen. We observed that progesterone was required for a T\u2010cell response following ivag immunization, whereas estradiol prevented a response. Although i.n. immunization stimulated OVA\u2010specific CD4<jats:sup>+<\/jats:sup> T\u2010cell responses in the draining LNs, it was substantially less effective compared to ivag. More importantly, an ivag booster immunization was absolutely required to attract T cells to the genital tract mucosa itself. While clinical use of CT is precluded because of its toxicity, we developed a combined adjuvant vector based on a non\u2010toxic derivative of CT and immune\u2010stimulating complexes. The CTA1\u2010DD\/immune\u2010stimulating complexes (ISCOMs) adjuvant together with major outer membrane protein was effective at stimulating genital tract CD4<jats:sup>+<\/jats:sup> T\u2010cell immunity and protection against a live chlamydial infection, which holds promise for the development of mucosal vaccines against sexually transmitted infections.<\/jats:p>","DOI":"10.1002\/eji.201041297","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T22:03:50Z","timestamp":1308348230000},"page":"2642-2653","source":"Crossref","is-referenced-by-count":26,"title":["CD4<sup>+<\/sup> T\u2010cell immunity in the female genital tract is critically dependent on local mucosal immunization"],"prefix":"10.1002","volume":"41","author":[{"given":"Ellen","family":"Marks","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Helgeby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan O.","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Sch\u00f6n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nils Y.","family":"Lycke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,8,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2001.01167.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2010.06.155"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0298"},{"key":"e_1_2_6_5_2","doi-asserted-by":"crossref","first-page":"3302","DOI":"10.1128\/iai.63.9.3302-3308.1995","article-title":"CD4+T cells play a significant role in adoptive immunity to Chlamydia trachomatis infection of the mouse genital tract","volume":"63","author":"Su H.","year":"1995","journal-title":"Infect. 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