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This antibody has been shown to deplete not only neutrophils but also monocytes and a subset of CD8<jats:sup>+<\/jats:sup> T cells. Recently, an antibody against Ly6G, which specifically depletes neutrophils, was characterized. In the present study, neutrophils are depleted using the antibody against Ly6G during infection with the intracellular bacterium <jats:italic>Listeria monocytogenes<\/jats:italic> (LM). Our data show that neutrophil\u2010depleted mice are much less susceptible to infection than mice depleted with anti\u2010Gr\u20101. Although neutrophils are required for clearance of LM, their importance is more pronounced in the liver and during a high\u2010dose bacterial challenge. Furthermore, we demonstrate that the protection mediated by neutrophils is due to the production of TNF\u2010\u03b1, but not IFN\u2010\u03b3. Additionally, neutrophils are not required for the recruitment of monocytes or the generation of adaptive T\u2010cell responses during LM infection. This study highlights the importance of neutrophils during LM infection, and indicate that depletion of neutrophils is less detrimental to the host than depletion of all Gr\u20101\u2010expressing cell populations.<\/jats:p>","DOI":"10.1002\/eji.201041363","type":"journal-article","created":{"date-parts":[[2011,6,10]],"date-time":"2011-06-10T09:57:31Z","timestamp":1307699851000},"page":"2666-2676","source":"Crossref","is-referenced-by-count":121,"title":["Specific depletion reveals a novel role for neutrophil\u2010mediated protection in the liver during <i>Listeria monocytogenes<\/i> infection"],"prefix":"10.1002","volume":"41","author":[{"given":"Karen D.","family":"Carr","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy N.","family":"Sieve","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohanalaxmi","family":"Indramohan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J.","family":"Break","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suheung","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rance E.","family":"Berg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,8,3]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1461"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0407247"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.151.5.2399"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.1.259"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.156.2.679"},{"key":"e_1_2_6_7_2","doi-asserted-by":"crossref","first-page":"127","DOI":"10.4049\/jimmunol.143.1.127","article-title":"Tumor necrosis factor is involved in the T cell\u2010independent pathway of macrophage activation in scid mice","volume":"143","author":"Bancroft G. 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