{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T08:21:51Z","timestamp":1743063711618},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,12,5]],"date-time":"2011-12-05T00:00:00Z","timestamp":1323043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Severely burned mice are susceptible to sepsis stemming from <jats:italic>Enterococcus faecalis<\/jats:italic> translocation due to the impaired generation of M1 macrophages (M1M\u03d5s) in local translocation sites. In our previous studies, CCL2 has been characterized as a major effector molecule on the burn\u2010associated generation of M2M\u03d5s, an inhibitor cell type for resident M\u03d5 conversion into M1M\u03d5s. In this study, we tried to protect burned mice orally infected with <jats:italic>E. faecalis<\/jats:italic> utilizing CCL2 antisense oligodeoxynucleotides (ODNs). We show that M2M\u03d5s in mesenteric lymph nodes (MLNs) were not demonstrated in burned mice treated with CCL2 antisense ODNs. M1M\u03d5s were not induced by heat\u2010killed <jats:italic>E. faecalis<\/jats:italic> from resident M\u03d5s transwell\u2010cultured with mesenteric lymph node macrophages (MLN\u2010M\u03d5s) from burned mice, while M1M\u03d5s were induced by the same antigen from resident M\u03d5s transwell\u2010cultured with M\u03d5s which were isolated from burned mice treated with CCL2 antisense ODNs. Bacterial growth in MLNs was shown in burned mice orally infected with a lethal dose of <jats:italic>E. faecalis<\/jats:italic>. However, after the same infection, sepsis did not develop in burned mice treated with CCL2 antisense ODNs. These results indicate that bacterial translocation and subsequent sepsis are controlled in burned mice orally infected with a lethal dose of <jats:italic>E. faecalis<\/jats:italic> by gene therapy utilizing CCL2 antisense ODNs.<\/jats:p>","DOI":"10.1002\/eji.201141572","type":"journal-article","created":{"date-parts":[[2011,10,17]],"date-time":"2011-10-17T08:59:22Z","timestamp":1318841962000},"page":"158-164","source":"Crossref","is-referenced-by-count":9,"title":["Effect of CCL2 antisense oligodeoxynucleotides on bacterial translocation and subsequent sepsis in severely burned mice orally infected with <i>Enterococcus 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