{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:56:26Z","timestamp":1790398586720,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,12,12]],"date-time":"2011-12-12T00:00:00Z","timestamp":1323648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The suppression of inappropriate immune responses by Treg cells is one of the major ways that the body maintains immune tolerance and homeostasis. Since defects in the suppressive capacity of Treg cells underlie many different immune\u2010mediated diseases, there is great interest in developing ways to track the number and function of Treg cells as biomarkers of tolerance and in devising ways to enhance their function therapeutically. However, the methods of studying human Treg cells are fraught with technical challenges that can often lead to misinterpretation. The most common way to determine the suppressive capacity of human Treg cells is to measure their ability to suppress the proliferation of responding CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells. Here, we discuss the technical considerations that must be taken into account when performing suppression of T\u2010cell proliferation assays with human Treg cells. We also consider how T cells may falsely appear suppressive because of dying cells in the system, improper resting of T\u2010cell lines prior to the assay, or insufficient proliferation of the responding T cells. We propose that, in the future, classification of a population of cells as \u201cregulatory\u201d should rely on more than a simple test for blockade of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell proliferation.\n                  <\/jats:p>","DOI":"10.1002\/eji.201141651","type":"journal-article","created":{"date-parts":[[2011,12,12]],"date-time":"2011-12-12T05:41:22Z","timestamp":1323668482000},"page":"27-34","source":"Crossref","is-referenced-by-count":137,"title":["Suppression assays with human T regulatory cells: A technical guide"],"prefix":"10.1002","volume":"42","author":[{"given":"Alicia N.","family":"McMurchy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan 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