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We used flow cytometric analysis, BrdU labeling and our mathematical models of B\u2010cell population dynamics, to analyze how Lyn deficiency impacts B\u2010cell maturation and survival. We found that Lyn\u2010deficient transitional 1 (T1) cells develop normally, but T2 cells develop primarily from the T1 subset in the spleen and fail to also develop directly from BM immature B cells. Lyn\u2010deficient T2 cells either mature to the follicular B\u2010cell type at a close to normal rate, or die in this compartment rather than access the T3 anergic subset. The \u223c40% of WT follicular cells that were short\u2010lived exited primarily by joining the T3 anergic subset, whereas the \u223c15% Lyn<jats:sup>\u2212\/\u2212<\/jats:sup> follicular cells that were not long lived had a high death rate and died in this compartment rather than entering the T3 subset. We hypothesize that exaggerated BCR signaling resulting from weak interactions with self\u2010antigens is largely responsible for these alterations in Lyn\u2010deficient B cells.<\/jats:p>","DOI":"10.1002\/eji.201141940","type":"journal-article","created":{"date-parts":[[2011,11,7]],"date-time":"2011-11-07T07:32:25Z","timestamp":1320651145000},"page":"511-521","source":"Crossref","is-referenced-by-count":16,"title":["Lyn deficiency affects B\u2010cell maturation as well as survival"],"prefix":"10.1002","volume":"42","author":[{"given":"Gitit","family":"Shahaf","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew J.","family":"Gross","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michal","family":"Sternberg\u2010Simon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah","family":"Kaplan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony L.","family":"DeFranco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramit","family":"Mehr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,12,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"1970","DOI":"10.4049\/jimmunol.152.4.1970","article-title":"Deletion and editing of B\u2010cells that express antibodies to DNA","volume":"152","author":"Chen C.","year":"1994","journal-title":"J. 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