{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T11:23:10Z","timestamp":1761823390801},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2011,10,26]],"date-time":"2011-10-26T00:00:00Z","timestamp":1319587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The desire to induce HIV\u20101\u2010specific responses soon after birth to prevent breast milk transmission of HIV\u20101 led us to propose a vaccine regimen which primes HIV\u20101\u2010specific T cells using a recombinant <jats:italic>Mycobacterium bovis<\/jats:italic> bacillus Calmette\u2010Gu\u00e9rin (rBCG) vaccine. Because attenuated live bacterial vaccines are typically not sufficiently immunogenic as stand\u2010alone vaccines, rBCG\u2010primed T cells will likely require boost immunization(s). Here, we compared modified Danish (AERAS\u2010401) and Pasteur lysine auxotroph (222) strains of BCG expressing the immunogen HIVA for their potency to prime HIV\u20101\u2010specific responses in adult BALB\/c mice and examined four heterologous boosting HIVA vaccines for their immunogenic synergy. We found that both BCG.HIVA<jats:sup>401<\/jats:sup> and BCG.HIVA<jats:sup>222<\/jats:sup> primed HIV\u20101\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2010cell\u2010mediated responses. The strongest boosts were delivered by human adenovirus\u2010vectored HAdV5.HIVA and sheep atadenovirus\u2010vectored OAdV7.HIVA vaccines, followed by poxvirus MVA.HIVA; the weakest was plasmid pTH.HIVA DNA. The prime\u2010boost regimens induced T cells capable of efficient in vivo killing of sensitized target cells. We also observed that the BCG.HIVA<jats:sup>401<\/jats:sup> and BCG.HIVA<jats:sup>222<\/jats:sup> vaccines have broadly similar immunologic properties, but display a number of differences mainly detected through distinct profiles of soluble intercellular signaling molecules produced by immune splenocytes in response to both HIV\u20101\u2010 and BCG\u2010specific stimuli. These results encourage further development of the rBCG prime\u2010boost regimen.<\/jats:p>","DOI":"10.1002\/eji.201141962","type":"journal-article","created":{"date-parts":[[2011,9,19]],"date-time":"2011-09-19T16:08:38Z","timestamp":1316448518000},"page":"3542-3552","source":"Crossref","is-referenced-by-count":26,"title":["Optimizing HIV\u20101\u2010specific CD8<sup>+<\/sup> T\u2010cell induction by recombinant BCG in prime\u2010boost regimens with heterologous viral vectors"],"prefix":"10.1002","volume":"41","author":[{"given":"Richard","family":"Hopkins","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Bridgeman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Bourne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice","family":"Mbewe\u2010Mvula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerald C.","family":"Sadoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerald W.","family":"Both","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan","family":"Joseph","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Fulkerson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom\u00e1\u0161","family":"Hanke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,10,26]]},"reference":[{"key":"e_1_2_6_2_2","unstructured":"AIDS Epidemic update. 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