{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T20:46:58Z","timestamp":1762375618857},"reference-count":36,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,9,10]],"date-time":"2012-09-10T00:00:00Z","timestamp":1347235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p>Cross\u2010presentation is the pathway by which exogenous antigens are routed for presentation by <jats:styled-content style=\"fixed-case\">MHC<\/jats:styled-content> class I molecules leading to activation of antiviral <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell responses. However, there is little information describing the modulation of cross\u2010presentation and the impact of pathogen\u2010derived signals associated with <jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>apanese encephalitis virus (<jats:styled-content style=\"fixed-case\">JEV<\/jats:styled-content>), which is one of the most common causes of encephalitis in humans. In this study, we demonstrate that <jats:styled-content style=\"fixed-case\">JEV<\/jats:styled-content> infection could suppress in vivo cross\u2010presentation of soluble and cell\u2010associated antigens, thereby generating weak <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell responses to exogenous antigens, as evaluated by <jats:styled-content style=\"fixed-case\">CFSE<\/jats:styled-content> dilution of adoptively transferred <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells and in vivo <jats:styled-content style=\"fixed-case\">CTL<\/jats:styled-content> killing activity. Furthermore, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8\u03b1<jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>11c<jats:sup>+<\/jats:sup> dendritic cells (<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s), which are known to be far more efficient at cross\u2010presenting soluble antigens, played a specific role in contributing to <jats:styled-content style=\"fixed-case\">JEV<\/jats:styled-content>\u2010mediated inhibition of the cross\u2010presentation of exogenous antigens through interference with effective antigen uptake. Finally, this study provides evidence that <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>2\u2010<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>y<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>88 and p38 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> signal pathway might be involved in <jats:styled-content style=\"fixed-case\">JEV<\/jats:styled-content>\u2010mediated inhibition of cross\u2010presentation of soluble and cell\u2010associated antigens. These observations suggest that the modulation of cross\u2010presentation of exogenous antigens through <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content> signaling has important implications for antiviral immune responses against <jats:styled-content style=\"fixed-case\">JEV<\/jats:styled-content> infection and the development of effective vaccination strategies.<\/jats:p>","DOI":"10.1002\/eji.201142052","type":"journal-article","created":{"date-parts":[[2012,6,18]],"date-time":"2012-06-18T08:33:55Z","timestamp":1340008435000},"page":"2655-2666","source":"Crossref","is-referenced-by-count":24,"title":["Impaired cross\u2010presentation of <scp>CD<\/scp>8\u03b1<sup>+<\/sup><scp>CD<\/scp>11c<sup>+<\/sup> dendritic cells by <scp>J<\/scp>apanese encephalitis virus in a <scp>TLR<\/scp>2\/<scp>M<\/scp>y<scp>D<\/scp>88 signal pathway\u2010dependent manner"],"prefix":"10.1002","volume":"42","author":[{"given":"Abi G.","family":"Aleyas","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine and Bio\u2010Safety Research Institute Chonbuk National University  Jeonju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Woo","family":"Han","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Bio\u2010Safety Research Institute Chonbuk National University  Jeonju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajit Mahadev","family":"Patil","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Bio\u2010Safety Research Institute Chonbuk National University  Jeonju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong Bum","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Bio\u2010Safety Research Institute Chonbuk National University  Jeonju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koanhoi","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine Pusan National University  Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong Kug","family":"Eo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Bio\u2010Safety Research Institute Chonbuk National University  Jeonju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,9,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2008.3"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2010.01.022"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2011.04411.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2002.01513.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-146290"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.4.1996"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1164206"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20100348"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.54.110807.090510"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-46.2.659"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra030476"},{"key":"e_1_2_7_13_1","first-page":"1101","volume-title":"Fields virology","author":"Lindenbach B. 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