{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:51:30Z","timestamp":1761663090189},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2012,9]]},"abstract":"Heparin\u2010binding haemagglutinin (HBHA<\/jats:styled-content>)\u2010specific immune responses have been linked to protection against tuberculosis (TB<\/jats:styled-content>). We investigated the hypothesis that BCG<\/jats:styled-content> vaccination of human infants primes an HBHA<\/jats:styled-content>\u2010specific response, using multiplex to measure secreted cytokines and chemokines following HBHA<\/jats:styled-content> and Mycobacterium tuberculosis<\/jats:italic> purified protein derivative (PPD<\/jats:styled-content>) stimulation of diluted whole blood samples from BCG<\/jats:styled-content>\u2010vaccinated or \u2010unvaccinated infants. Of 42 analytes measured, 24 and 32 significant, BCG<\/jats:styled-content>\u2010associated increases were detected in response to HBHA<\/jats:styled-content> and PPD<\/jats:styled-content>, respectively. Both response profiles included T<\/jats:styled-content>h\u20101, T<\/jats:styled-content>h\u20102, T<\/jats:styled-content>h\u201017 and inflammatory cytokines and chemokines (e.g. IFN<\/jats:styled-content>\u2010\u03b3, TNF<\/jats:styled-content>\u2010\u03b1, IL<\/jats:styled-content>\u20105, IL<\/jats:styled-content>\u201010, IL<\/jats:styled-content>\u201013, IL<\/jats:styled-content>\u201017, MIP<\/jats:styled-content>\u20101\u03b1 and MIP<\/jats:styled-content>\u20101\u03b2). We also found that six of the seven responses most closely correlated with IFN<\/jats:styled-content>\u2010\u03b3 were common to both HBHA<\/jats:styled-content> and PPD<\/jats:styled-content>. Notably, all HBHA<\/jats:styled-content>\u2010specific secretion of cytokines and chemokines from infant samples was dependant on previous BCG<\/jats:styled-content> vaccination. Also, long\u2010term persistence of HBHA<\/jats:styled-content>\u2010specific responses was found in adolescents with evidence of infant BCG<\/jats:styled-content> vaccination. This study demonstrates for the first time BCG<\/jats:styled-content> priming of an HBHA<\/jats:styled-content>\u2010specific immune response in infants that is characterised by a broad cytokine and chemokine signature. It also suggests a number of BCG<\/jats:styled-content> vaccination associated, HBHA<\/jats:styled-content>\u2010induced responses that should be useful for future studies of biomarkers of protection against TB<\/jats:styled-content>.<\/jats:p>","DOI":"10.1002\/eji.201142297","type":"journal-article","created":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T09:53:31Z","timestamp":1338544411000},"page":"2511-2522","source":"Crossref","is-referenced-by-count":18,"title":["Broad heparin\u2010binding haemagglutinin\u2010specific cytokine and chemokine response in infants following Mycobacterium bovis BCG<\/scp> vaccination"],"prefix":"10.1002","volume":"42","author":[{"given":"Steven G.","family":"Smith","sequence":"first","affiliation":[{"name":"Faculty of Infectious and Tropical Diseases Department of Immunology and Infection London School of Hygiene & Tropical Medicine London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Lecher","sequence":"additional","affiliation":[{"name":"INSERM U1019 Lille France"},{"name":"CNRS UMR 8204 Lille France"},{"name":"Institut Pasteur de Lille, Center for Infection and Immunity of Lille Lille France"},{"name":"University Lille Nord de France Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rose","family":"Blitz","sequence":"additional","affiliation":[{"name":"Faculty of Infectious and Tropical Diseases Department of Immunology and Infection London School of Hygiene & Tropical Medicine London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camille","family":"Locht","sequence":"additional","affiliation":[{"name":"INSERM U1019 Lille France"},{"name":"CNRS UMR 8204 Lille France"},{"name":"Institut Pasteur de Lille, Center for Infection and Immunity of Lille Lille France"},{"name":"University Lille Nord de France Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hazel M.","family":"Dockrell","sequence":"additional","affiliation":[{"name":"Faculty of Infectious and Tropical Diseases Department of Immunology and Infection London School of Hygiene & Tropical Medicine London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.4.982"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1542\/peds.96.1.29","article-title":"The efficacy of bacillus Calmette\u2010Guerin vaccination of newborns and infants in the prevention of tuberculosis: meta\u2010analyses of the published literature","volume":"96","author":"Colditz G. A.","year":"1995","journal-title":"Pediatrics"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68507-3"},{"key":"e_1_2_7_5_1","first-page":"1171","article-title":"Neonatal BCG protection against tuberculosis lasts for 20 years in Brazil","volume":"9","author":"Barreto M. L.","year":"2005","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_2_7_6_1","first-page":"536","article-title":"A case\u2010control study of protection against tuberculosis by BCG revaccination in Recife, Brazil","volume":"10","author":"Dantas O. M.","year":"2006","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1128"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200737504"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.3.993"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/35084083"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1086\/338833"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1086\/430930"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200012)30:12<3689::AID-IMMU3689>3.0.CO;2-4"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1090"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.04.027"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.07.005"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/730702"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.11.004"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000552"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.06.103"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000926"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-11-35"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.5.3569"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201141841"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1086\/597069"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-0059-9_24"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.2001.1819"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.6.2249"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.6.2243"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)08353-8"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2007.04.005"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2004.08.006"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2128-2137.2006"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703510104"},{"key":"e_1_2_7_35_1","unstructured":"Anderson L. Moore J. Kruijshaar M. Pedrazzoli D. Bradshaw L. Crofts J. Stagg H.et\u00a0al. Tuberculosis in theUK: annual report on tuberculosis surveillance in theUK 2010.Health Protection Agency Centre for Infections London October2010."},{"key":"e_1_2_7_36_1","unstructured":"Anderson C. Maguire H. Anderson S. Carless J.andKanfoudi L. Tuberculosis inLondon 2009: annual report on tuberculosis surveillance inLondon.Health Protection Agency London Regional Epidemiology Unit London January2011."}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.201142297","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.201142297","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T12:56:39Z","timestamp":1695473799000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.201142297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8]]},"references-count":35,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["10.1002\/eji.201142297"],"URL":"https:\/\/doi.org\/10.1002\/eji.201142297","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8]]}}}