{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:32:50Z","timestamp":1791999170463,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,8,6]],"date-time":"2012-08-06T00:00:00Z","timestamp":1344211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:p>Here, we have used primary vaccination of healthy donors with attenuated live yellow fever virus 17<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">YFV<\/jats:styled-content>\u201017<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>) as a model to study the generation of protective immunity. In short intervals after vaccination, we analyzed the induction of <jats:styled-content style=\"fixed-case\">YFV<\/jats:styled-content>\u201017<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content> specific <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010 and <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cell immunity, bystander activation, dendritic cell subsets, changes in serum cytokine levels, and <jats:styled-content style=\"fixed-case\">YFV<\/jats:styled-content>\u201017<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>\u2010specific antibodies. We show activation of innate immunity and a concomitant decline of numbers of peripheral blood <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells. An early peak of antigen\u2010specific <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells at day 2, followed by mobilization of innate immune cells, preceded the development of maximal adaptive immunity against <jats:styled-content style=\"fixed-case\">YFV<\/jats:styled-content>\u201017<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content> at day 14 after vaccination. Interestingly, potent adaptive immunity as measured by high titers of neutralizing <jats:styled-content style=\"fixed-case\">YFV<\/jats:styled-content>\u201017<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>\u2010specific antibodies, correlated with early activation and recruitment of <jats:styled-content style=\"fixed-case\">YFV<\/jats:styled-content>\u201017<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>\u2010specific <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells and higher levels of s<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>. Thus our data might provide new insights into the interplay of innate and adaptive immunity for the induction of protective immunity.<\/jats:p>","DOI":"10.1002\/eji.201142306","type":"journal-article","created":{"date-parts":[[2012,6,26]],"date-time":"2012-06-26T14:08:11Z","timestamp":1340719691000},"page":"2363-2373","source":"Crossref","is-referenced-by-count":67,"title":["The early cellular signatures of protective immunity induced by live viral vaccination"],"prefix":"10.1002","volume":"42","author":[{"given":"Siegfried","family":"Kohler","sequence":"first","affiliation":[{"name":"Charit\u00e9 University Medicine CCM Klinik f\u00fcr Neurologie\/NCRC  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Bethke","sequence":"additional","affiliation":[{"name":"Charit\u00e9 University Medicine CVK  Berlin 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