{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:53:29Z","timestamp":1761155609485},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,8,6]],"date-time":"2012-08-06T00:00:00Z","timestamp":1344211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2012,10]]},"abstract":"It is known that overlapping HIV<\/jats:styled-content>\u20101 peptides of different lengths can be presented by a given HLA<\/jats:styled-content> class I molecule. However, the role of those peptides in CD<\/jats:styled-content>8+<\/jats:sup> T cells recognition of HIV<\/jats:styled-content>\u20101\u2010infected cells remains unclear. Here we investigated the recognition of overlapping 8\u2010mer to 11\u2010mer peptides of Pol 155\u2013165 by HLA<\/jats:styled-content>\u2010B*54:01\u2010restricted CD<\/jats:styled-content>8+<\/jats:sup> T cells. The analysis of ex vivo T cells using ELISPOT and tetramer binding assays showed that there were different patterns of CD<\/jats:styled-content>8+<\/jats:sup> T\u2010cell responses to these peptides among chronically HIV<\/jats:styled-content>\u20101\u2010infected HLA<\/jats:styled-content>\u2010B*54:01+<\/jats:sup> individuals, though the response to the 9\u2010mer peptide was the strongest among them. CD<\/jats:styled-content>8+<\/jats:sup> T\u2010cell clones with TCRs specific for the 9\u2010mer, 10\u2010mer, and\/or 11\u2010mer peptides effectively killed HIV<\/jats:styled-content>\u20101\u2010infected cells. Together, these results suggest that the 9\u2010mer and 10\u2010mer peptides could be predominantly presented by HLA<\/jats:styled-content>\u2010B*54:01, though it remains possible that the 11\u2010mer peptide was also presented by this HLA<\/jats:styled-content> allele. The present study demonstrates effective CD<\/jats:styled-content>8+<\/jats:sup> T\u2010cell recognition of HIV<\/jats:styled-content>\u20101\u2010infected cells via presentation of multiple overlapping HIV<\/jats:styled-content>\u20101 peptides and cross\u2010recognition by the CD<\/jats:styled-content>8+<\/jats:sup> T<\/jats:styled-content> cells.<\/jats:p>","DOI":"10.1002\/eji.201242483","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T04:59:11Z","timestamp":1340859551000},"page":"2621-2631","source":"Crossref","is-referenced-by-count":6,"title":["CTL<\/scp> recognition of HIV<\/scp>\u20101\u2010infected cells via cross\u2010recognition of multiple overlapping peptides from a single 11\u2010mer P<\/scp>ol sequence"],"prefix":"10.1002","volume":"42","author":[{"given":"Masao","family":"Hashimoto","sequence":"first","affiliation":[{"name":"Center for AIDS Research Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiro","family":"Akahoshi","sequence":"additional","affiliation":[{"name":"Center for AIDS Research Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayato","family":"Murakoshi","sequence":"additional","affiliation":[{"name":"Center for AIDS Research Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Ishizuka","sequence":"additional","affiliation":[{"name":"Sanofi\u2010Aventis Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichi","family":"Oka","sequence":"additional","affiliation":[{"name":"Center for AIDS Research Kumamoto University Kumamoto Japan"},{"name":"AIDS Clinical Center National Center for Global Health and Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masafumi","family":"Takiguchi","sequence":"additional","affiliation":[{"name":"Center for AIDS Research Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,8,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070784"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.f.1774"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01754-06"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1143767"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1333"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2357"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.10.7406"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00171-10"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni845"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1195271"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05115"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01452-07"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.10.6164"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.11.5291-5299.2000"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"6242","DOI":"10.4049\/jimmunol.159.12.6242","article-title":"Identification and characterization of multiple HLA\u2010A24\u2010restricted HIV\u20101 CTL epitopes: strong epitopes are derived from V regions of HIV\u20101","volume":"159","author":"Ikeda\u2010Moore Y.","year":"1997","journal-title":"J. Immunol."},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI32047"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.2.239"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803471"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1728"},{"key":"e_1_2_7_21_1","doi-asserted-by":"crossref","first-page":"5026","DOI":"10.4049\/jimmunol.158.10.5026","article-title":"Evidence of presentation of multiple HIV\u20101 cytotoxic lymphocyte epitopes by HLA\u2010B*3501 molecules that are associated with the accelerated progression to AIDS","volume":"158","author":"Tomiyama H.","year":"1997","journal-title":"J. Immunol."},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.2.1107"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.2000.560606.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2008.04.006"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(95)00039-X"},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"3480","DOI":"10.4049\/jimmunol.157.8.3480","article-title":"Specificity and degeneracy in peptide binding to HLA\u2010B7\u2010like class I molecules","volume":"157","author":"Sidney J.","year":"1996","journal-title":"J. Immunol."},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3084"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2009.10.015"},{"key":"e_1_2_7_29_1","doi-asserted-by":"crossref","first-page":"2804","DOI":"10.4049\/jimmunol.128.6.2804","article-title":"A monoclonal antibody directed against the HLA\u2010Bw6 epitope","volume":"128","author":"Radka S. F","year":"1982","journal-title":"J. Immunol."},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.59.2.284-291.1986"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5284.94"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.201242483","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.201242483","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T21:26:29Z","timestamp":1695504389000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.201242483"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,6]]},"references-count":30,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2012,10]]}},"alternative-id":["10.1002\/eji.201242483"],"URL":"https:\/\/doi.org\/10.1002\/eji.201242483","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,6]]}}}