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We have studied the functions of <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>karos in the transition from pre\u2010<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cell to the <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> thymocyte using an Ikaros null <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>\u2212<\/jats:sup><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>\u2212<\/jats:sup> mouse thymoma cell line (<jats:styled-content style=\"fixed-case\">JE<\/jats:styled-content>131). We demonstrate that this cell line carries a single functional <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content> \u03b2 gene rearrangement and expresses a surface pre\u2010<jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">JE<\/jats:styled-content>131 cells also carry nonfunctional rearrangements on both alleles of their <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content> \u03b1 loci. Retroviral reintroduction of <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>karos dramatically increased the rate of transcription in the \u03b1 locus and <jats:styled-content style=\"fixed-case\">TCR V<\/jats:styled-content>\u03b1\/<jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>\u03b1 recombination resulting in the appearance of many new \u03b1\u03b2<jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content><jats:sup>+<\/jats:sup> cells. The process is <jats:styled-content style=\"fixed-case\">RAG<\/jats:styled-content> dependent, requires switch\/sucrose nonfermentable chromatin\u2010remodeling complexes and is coincident with the binding of <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>karos to the <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content> \u03b1 enhancer. Furthermore, knockdown of <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>i2\/nucleosome remodeling and deacetylase complexes increased the frequency of <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content> \u03b1 rearrangement. Our data suggest that <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>karos controls <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>\u03b1\/<jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>\u03b1 recombination in <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells by controlling access of the transcription and recombination machinery to the <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content> \u03b1 loci. The <jats:styled-content style=\"fixed-case\">JE<\/jats:styled-content>131 cell line should prove to be a very useful tool for studying the molecular details of this and other processes involved in the pre\u2010<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cell to \u03b1\u03b2<jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content><jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> thymocyte transition.<\/jats:p>","DOI":"10.1002\/eji.201242757","type":"journal-article","created":{"date-parts":[[2012,11,21]],"date-time":"2012-11-21T13:21:27Z","timestamp":1353504087000},"page":"521-532","source":"Crossref","is-referenced-by-count":14,"title":["<scp>I<\/scp>karos promotes rearrangement of <scp>TCR<\/scp> \u03b1 genes in an <scp>I<\/scp>karos null thymoma cell line"],"prefix":"10.1002","volume":"43","author":[{"given":"Bernard","family":"Collins","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute National Jewish Health  Denver CO USA"},{"name":"Integrated Department of Immunology National Jewish Health  Denver CO USA"},{"name":"Department of Biochemistry and Molecular Genetics, University of Colorado Denver Anschutz Medical Campus  Aurora CO USA"},{"name":"Program in Biomolecular Structure University of Colorado Denver Anschutz Medical Campus  Aurora CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric T.","family":"Clambey","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute National Jewish Health  Denver CO USA"},{"name":"Integrated Department of Immunology National Jewish Health  Denver CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Scott\u2010Browne","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute National Jewish Health  Denver CO USA"},{"name":"Integrated Department of Immunology National Jewish Health  Denver CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice","family":"White","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute National Jewish Health  Denver CO USA"},{"name":"Integrated Department of Immunology National Jewish Health  Denver CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippa","family":"Marrack","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute National Jewish Health  Denver CO USA"},{"name":"Integrated Department of Immunology National Jewish Health  Denver CO USA"},{"name":"Department of Biochemistry and Molecular Genetics, University of Colorado Denver Anschutz Medical Campus  Aurora CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Hagman","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology National Jewish Health  Denver CO USA"},{"name":"Department of Biochemistry and Molecular Genetics, University of Colorado Denver Anschutz Medical Campus  Aurora CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Kappler","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute National Jewish Health  Denver CO USA"},{"name":"Integrated Department of Immunology National Jewish Health  Denver CO USA"},{"name":"Program in Biomolecular Structure University of Colorado Denver Anschutz Medical Campus  Aurora CO 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