{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:07:30Z","timestamp":1761919650955},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,18]],"date-time":"2013-01-18T00:00:00Z","timestamp":1358467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:p>To test whether mechanisms controlling the range of diversity of the developing antibody repertoire in <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mice (<jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content><jats:sup>b<\/jats:sup>) operate similarly to those identified in <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice (<jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content><jats:sup>a<\/jats:sup>), we compared the sequences of <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content><jats:sub>H<\/jats:sub>7183\u2010containing <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>\u2010chain transcripts from sorted adult bone marrow <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cell subsets with those previously obtained from <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice. Patterns of <jats:styled-content style=\"fixed-case\">VDJ<\/jats:styled-content> gene segment utilization and <jats:styled-content style=\"fixed-case\">CDR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>3 amino acid composition, charge, and average length in <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 pro\u2010<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells were similar, although not identical, to <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c pro\u2010<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells. However, <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mature, recirculating <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells failed to demonstrate the reduction in the use of <jats:styled-content style=\"fixed-case\">VH<\/jats:styled-content>81<jats:styled-content style=\"fixed-case\">X<\/jats:styled-content> and the narrowing in the range of variance of <jats:styled-content style=\"fixed-case\">CDR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>3 hydrophobicity that characterizes <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cell maturation in <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice. To further test the ability of the <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 strain to discard <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells expressing highly charged <jats:styled-content style=\"fixed-case\">CDR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>3s, we introduced a mutant <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content><jats:sup>a<\/jats:sup> <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content><jats:sub>H<\/jats:sub> allele that forces use of arginine, asparagine, and histidine. Unlike <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice, <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mice congenic for the charged <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content><jats:sub>H<\/jats:sub> maintained normal numbers of mature, recirculating <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells that were enriched for charged <jats:styled-content style=\"fixed-case\">CDR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>3s. Together these findings indicate that the mature <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cell pool permits expression of immunoglobulins with antigen\u2010binding sites that are typically discarded during late\u2010stage bone marrow <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cell development in <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice.<\/jats:p>","DOI":"10.1002\/eji.201242936","type":"journal-article","created":{"date-parts":[[2012,12,6]],"date-time":"2012-12-06T13:55:36Z","timestamp":1354802136000},"page":"629-640","source":"Crossref","is-referenced-by-count":14,"title":["Recirculating bone marrow <scp>B<\/scp> cells in <scp>C<\/scp>57<scp>BL<\/scp>\/6 mice are more tolerant of highly hydrophobic and highly charged <scp>CDR<\/scp>\u2010<scp>H<\/scp>3s than those in <scp>BALB<\/scp>\/c mice"],"prefix":"10.1002","volume":"43","author":[{"given":"Mohamed","family":"Khass","sequence":"first","affiliation":[{"name":"Department of Microbiology University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Buckley","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Wake Forest University School of Medicine Winston\u2010Salem NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pratibha","family":"Kapoor","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Schelonka","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Oregon Health &amp; Science University Portland OR USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leticia S.","family":"Watkins","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingxin","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Harry W.","family":"Schroeder","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Alabama at Birmingham Birmingham AL USA"},{"name":"Department of Medicine University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/302575a0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/381751a0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05830.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.13.4118"},{"key":"e_1_2_7_6_1","volume-title":"Sequences of proteins of immunological interest","author":"Kabat E. 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