{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:05:23Z","timestamp":1792663523709,"version":"4.5.0"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,3,6]],"date-time":"2013-03-06T00:00:00Z","timestamp":1362528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:p>\n                    Plasmacytoid pre\u2010dendritic cells (pDCs) are specialized in responding to nucleic acids, and link innate with adaptive immunity. Although the response of pDCs to viruses is well established, whether pDCs can respond to extracellular bacteria remains controversial. Here, we demonstrate that extracellular bacteria such as\n                    <jats:italic>Neisseria meningitidis<\/jats:italic>\n                    ,\n                    <jats:italic>Haemophilus influenzae<\/jats:italic>\n                    , and\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    activate pDCs to produce IFN\u2010\u03b1, TNF\u2010\u03b1, IL\u20106, and to upregulate CD86 expression. We observed that pDCs were present within tonsillar crypts and oro\u2010nasopharyngeal epithelium, where they may contact extracellular bacteria, in situ. Tonsil epithelium\u2010conditioned supernatants inhibited IFN\u2010\u03b1, TNF\u2010\u03b1, and IL\u20106 triggered by the direct contact of\n                    <jats:italic>N. meningitidis<\/jats:italic>\n                    or\n                    <jats:italic>S. aureus<\/jats:italic>\n                    with pDCs. However, pDC priming of naive T cells was not affected, suggesting that tonsil epithelium micro\u2010environment limits local inflammation while preserving adaptive immunity in response to extracellular bacteria. Our results reveal an important and novel function of pDCs in the initiation of the mucosal innate and adaptive immunity to extracellular bacteria.\n                  <\/jats:p>","DOI":"10.1002\/eji.201242990","type":"journal-article","created":{"date-parts":[[2013,2,22]],"date-time":"2013-02-22T04:14:47Z","timestamp":1361506487000},"page":"1264-1273","source":"Crossref","is-referenced-by-count":45,"title":["Epithelial control of the human pDC response to extracellular bacteria"],"prefix":"10.1002","volume":"43","author":[{"given":"Paula","family":"Michea","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U932  Paris France"},{"name":"Institut Curie Department of Immunology  Paris France"},{"name":"INSERM U970 Paris Cardiovascular Research Centre  Paris France"},{"name":"Facult\u00e9 de M\u00e9decine Paris Descartes Universit\u00e9 Paris Descartes  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Vargas","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U932  Paris France"},{"name":"Institut Curie Department of Immunology  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010H\u00e9l\u00e8ne","family":"Donnadieu","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U932  Paris France"},{"name":"Institut Curie Department of Immunology  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Rosemblatt","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Ciencia y Vida  Santiago Chile"},{"name":"Facultad de Ciencias de la Salud Universidad Andr\u00e9s Bello  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Rosa","family":"Bono","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Facultad de Ciencias Universidad de Chile  Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Dum\u00e9nil","sequence":"additional","affiliation":[{"name":"INSERM U970 Paris Cardiovascular Research Centre  Paris France"},{"name":"Facult\u00e9 de M\u00e9decine Paris Descartes Universit\u00e9 Paris Descartes  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vassili","family":"Soumelis","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U932  Paris France"},{"name":"Institut Curie Department of Immunology  Paris 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